Agricultural and Veterinary Sciences

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Browsing Agricultural and Veterinary Sciences by Subject "16S rRNA, succession, compost, bacterial community, pyrosequencing."

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    Bacterial community changes during composting of municipal crop waste using low technology methods as revealed by 16S rRNA1* and Department
    (African Journal of Environmental Science and Technology, 2018) Tumuhairwe, John Baptist; Tenywa, John Stephen
    Bacterial communities are actively involved in composting process but the environment within the compost influences their diversity, abundance and succession. In this study, the authors investigated the bacterial communities in tropical crop waste compost using pyrosequencing. Municipal crop wastes from the tropics (Uganda) were composted under four different low-technology methods. Samples were collected from the early thermophilic, late thermophilic, and mesophilic phases, and from mature compost. Pyrosequencing of the amplified variable V4 region of the 16s rDNA generated over 110 000 sequences. Chao1 and cluster analysis at 3% dissimilarity showed that bacterial community richness declined during the composting process. The community was dominated by a few bacterial taxa during the thermophilic phases. Species evenness increased as compost progressed to maturity despite a decline in the number of taxa over the successional progression. Bacterial community diversity, abundance and succession changed with the composting method. This pattern of diversity may be attributed to competition and selection during the microbial succession. A total of 22 phyla and 513 genera were identified from all the methods in the entire composting process. The most abundant phyla were Proteobacteria, Firmicutes, Bacteriodes and Actinobacteria. Pyrosequencing provided more information on compost bacterial community diversity and abundance than previously used molecular methods. Several novel bacteria existing in tropical crop waste compost remained unclassified.