Browsing by Author "Zirintunda, Gerald"
Now showing 1 - 11 of 11
Results Per Page
Sort Options
Item An analysis of Heavy Metals Contamination and Estimating the Daily intakes of Vegetables from Uganda(Toxicology Research and Application, 2021) Kasozi, Keneth Iceland; Otim, Eric Oloya; Ninsiima, Herbert Izo; Zirintunda, Gerald; Tamale, Andrew; Musoke, Grace Henry; Muyinda, Robert; Ssempijja, FredEnvironmental contamination with elevated levels of copper (Cu), cobalt (Co), iron (Fe), zinc (Zn), lead (Pb), chromium (Cr6+), cadmium (Cd), and nickel (Ni)—all states of which are found in Uganda—raises health risk to the public. Pb, Cr6+, Cd, and Ni for instance are generally considered nonessential to cellular functions, notwithstanding the importance of the oxidative state of the metals in bioavailability. As such, we aimed in this study (i) to evaluate heavy metal concentrations in four vegetables from a typical open-air market in Uganda, (ii) to assess the safety of consuming these vegetables against the World Health Organization (WHO) recommended limits of heavy metals consumption, and (iii) to formulate a model of estimated daily intake (EDI) among consumers in the country.This was a cross-sectional study conducted in five georeferenced markets of Bushenyi district in January 2020. Amaranthus, cabbages, scarlet eggplants, and tomatoes were collected from open markets, processed, and analyzed by atomic absorption spectrometry. Modeled EDI, principal component (PCA) and cluster analysis (CA) were conducted to identify relationships in the samples.The levels of essential elements in the four vegetables were found to fall from Co > Cu > Fe > Zn. Those of non-essential metals were significantly higher and followed the pattern Cd > Cr > Pb > Ni. The highest EDI values were those of Cu in scarlet eggplants, Zn in amaranthus, Fe in amaranthus, Co in amaranthus, Pb in cabbages, total Cr in scarlet eggplant, Cd in cabbages and tomatoes, and Ni in cabbages. In comparison to international limits, EDIs for Zn, Cu, Co and Fe were low while Ni in cabbages were high. PCA showed high variations in scarlet eggplant and amaranthus. The study vegetables were found to be related with each other, not according to the location of the markets from where they were obtained, but according to their species by CA.The presence of non-essential elements above WHO limits raises policy challenges for the consumption and marketing of vegetables in the study area. Furthermore, low EDIs of essential elements in the vegetables create demand for nutritious foods to promote healthy communities.Item Bee Venom—A Potential Complementary Medicine Candidate for SARS-CoV-2 Infections(Frontiers in public health, 2020) Kasozi, Keneth Iceland; Alqarni, Mohammed; Zirintunda, Gerald; Ssempijja, Fred; Musinguzi, Simon Peter; Matama, Kevin; Mbiydzenyuy, Ngala Elvis; Welburn, Susan ChristinaSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is characterized by severe cytokine storm syndrome following inflammation. SARS-CoV-2 directly interacts with angiotensin-converting enzyme 2 (ACE-2) receptors in the human body. Complementary therapies that impact on expression of IgE and IgG antibodies, including administration of bee venom (BV), have efficacy in the management of arthritis, and Parkinson's disease. A recent epidemiological study in China showed that local beekeepers have a level of immunity against SARS-CoV-2 with and without previous exposure to virus. BV anti-inflammatory properties are associated with melittin and phospholipase A2 (PLA2), both of which show activity against enveloped and non-enveloped viruses, including H1N1 and HIV, with activity mediated through antagonist activity against interleukin-6 (IL-6), IL-8, interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α). Melittin is associated with the underexpression of proinflammatory cytokines, including nuclear factor-kappa B (NF-κB), extracellular signal-regulated kinases (ERK1/2), and protein kinase Akt. BV therapy also involves group III secretory phospholipase A2 in the management of respiratory and neurological diseases. BV activation of the cellular and humoral immune systems should be explored for the application of complementary medicine for the management of SARS-CoV-2 infections. BV “vaccination” is used to immunize against cytomegalovirus and can suppress metastases through the PLA2 and phosphatidylinositol-(3,4)-bisphosphate pathways. That BV shows efficacy for HIV and H1NI offers opportunity as a candidate for complementary therapy for protection against SARS-CoV-2.Item Descriptive Analysis of Heavy Metals Content of Beef From Eastern Uganda and Their Safety for Public Consumption(Frontiers in Nutrition, 2021) Kasozi, Keneth Iceland; Hamira, Yunusu; Zirintunda, Gerald; Tamale, Andrew; Matama, Kevin; Ssempijja, Fred; Muyinda, Robert; Kawooya, Francis; Kisakye, Hellen; Bogere, Paul; Matovu, Henry; Etiang, Patrick; Ochieng, Juma JohnIn this study, we initiated an effort to generate information about beef safety in Uganda. Our entry point was to assess by atomic absorption spectrophotometry the levels of essential elements copper (Cu), cobalt (Co), iron (Fe) and zinc (Zn), and non-essential elements lead (Pb), chromium (Cr), nickel (Ni), and cadmium (Cd) in 40 beef samples collected from within and around Soroti (Uganda). The information was used to evaluate the safety of consuming such beef against the World Health Organization (WHO) limits. The latter was accomplished by (i) estimating the daily intake (EDI) of each metal in the study area, (ii) modeling the non-cancer health risk using the target hazard quotient (THQ) and (iii) modeling the cancer risk using the incremental lifetime cancer risk (ILCR). The study finds that the mean concentrations (±95% CI) and EDI were in the order of Fe > Zn > Cr > Ni > Pb > Co > Cu > Cd. Cancer risk was found to be due to Ni > Cr > Cd > Pb and significantly higher in children than adults. The latter particularly demonstrates the importance of Ni poisoning in the study area. Overall, while essential elements in our beef samples were below WHO limits (hence no health risks), non-essential elements had high health and cancer risks due to higher levels of Cr and Ni.Item Effects of b-Blockers on the Sympathetic and Cytokines Storms in Covid-19(Frontiers in Immunology, 2021) Al-kuraishy, Hayder M.; Al-Gareeb, Ali Ismail; Kasozi, Keneth Iceland; Zirintunda, Gerald; Welburn, Susan Christina; Batiha, Gaber El-SaberSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a causative virus in the development of coronavirus disease 2019 (Covid-19) pandemic. Respiratory manifestations of SARS-CoV-2 infection such as acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) leads to hypoxia, oxidative stress, and sympatho-activation and in severe cases leads to sympathetic storm (SS). On the other hand, an exaggerated immune response to the SARS-CoV-2 invasion may lead to uncontrolled release of pro-inflammatory cytokine development of cytokine storm (CS). In Covid-19, there are interactive interactions between CS and SS in the development of multi-organ failure (MOF). Interestingly, cutting the bridge between CS and SS by anti-inflammatory and anti-adrenergic agents may mitigate complications that are induced by SARS-CoV-2 infection in severely affected Covid-19 patients. The potential mechanisms of SS in Covid-19 are through different pathways such as hypoxia, which activate the central sympathetic center through carotid bodies chemosensory input and induced pro-inflammatory cytokines, which cross the blood-brain barrier and activation of the sympathetic center. β2-receptors signaling pathway play a crucial role in the production of pro-inflammatory cytokines, macrophage activation, and B-cells for the production of antibodies with inflammation exacerbation. β-blockers have anti-inflammatory effects through reduction release of pro-inflammatory cytokines with inhibition of NF-κB. In conclusion, β-blockers interrupt this interaction through inhibition of several mediators of CS and SS with prevention development of neural-cytokine loop in SARS-CoV-2 infection. Evidence from this study triggers an idea for future prospective studies to confirm the potential role of β-blockers in the management of Covid-19.Item Embracing One Health offers practical strategies in management of COVID-19 for Africa(The Pan African Medical Journal, 2020) Kasozi, Keneth Iceland; Mujinya, Regan; Bogere, Paul; Ekou, Justine; Zirintunda, Gerald; Ahimbisibwe, Salaviriuse; Matama, Kevin; Ninsiima, Herbert Izo; Ayikobua, Emmanuel Tiyo; Ssimbwa, Godfrey; Musinguzi, Simon Peter; Muyinda, Robert; Ssempijja, Fred; Matovu, Henry; MacLeod, Ewan; Anderson, Neil Euan; Welburn, Susan ChristinaThe coronavirus, COVID-19 outbreak has now affected over 60% of African countries in less than two months , gaining a foothold through major economic and transport hubs on the African continent including Egypt, Algeria, Nigeria, South Africa and Kenya. Travel restrictions imposed against citizens from countries with major outbreaks including China, USA and those in Europe were too late . African Union member states as of early April 2020 are reporting 6,470 cases and 241 deaths from COVID-19 reporting growth as “close to exponential”. Africa Centers for Disease Control and Prevention acknowledges the virus is an existential threat to African countries and that with local transmission now underway many would pass the 10,000-infection mark by the end of April. While the impact of wearing of face masks for control of COVID-19 remains controversial, it is inarguable that respiratory transmission needs to be prevented. Currently, there is a global shortage of masks and personal protective equipment (PPE) and distribution is being rationed in developed countries to retain this for workers in the health system, showing that developing countries in Africa are bound to suffer more should the pandemic be mismanaged at these early stages. In addition, health systems in developing countries, already crippled from years of underinvestment will be compromised unless practical and realistic prevention strategies are put in place. China, Italy, France, UK and USA, all with sophisticated health systems, have found COVID-19 challenging. Infection is increasing across the African subcontinent and health systems will struggle as the pandemic sweeps into and across Africa.Item Emerging Anthelmintic Resistance in Poultry: Can Ethnopharmacological Approaches Offer a Solution?(Frontiers in pharmacology, 2021) Zirintunda, Gerald; Biryomumaisho, Savino; Kasozi, Keneth Iceland; Kateregga, John; Vudriko, Patrick; Nalule, Sarah; Olila, Deogracious; Kajoba, Mariam; Matama, Kevin; Kwizera, Mercy Rukundo; Abdelgawad, Mohamed A.; Acai-Okwee, JamesLimited pharmacological studies have been conducted on plant species used against poultry helminths. The objective of this study was to provide a basis for plant based anthelmintics as possible alternatives against poultry anthelmintic resistance. The study justified the need for alternative anthelmintics. The study places emphasis on the increasing anthelmintic resistance, mechanism of resistance, and preparational protocols for plant anthelmintics and their associated mechanism of action. Pharmaceutical studies on plants as alternative therapies for the control of helminth parasites have not been fully explored especially in several developing countries. Plants from a broad range of species produce a wide variety of compounds that are potential anthelmintics candidates. Important phenolic acids have been found in Brassica rapa L. and Terminalia avicenniodes Guill. and Perri that affect the cell signaling pathways and gene expression. Benzo (c) phenanthridine and isoquinoline alkaloids are neurotoxic to helminths. Steroidal saponins (polyphyllin D and dioscin) interact with helminthic mitochondrial activity, alter cell membrane permeability, vacuolation and membrane damage. Benzyl isothiocyanate glucosinolates interfere with DNA replication and protein expression, while isoflavones from Acacia oxyphylla cause helminth flaccid paralysis, inhibit energy generation, and affect calcium utilization. Condensed tannins have been shown to cause the death of nematodes and paralysis leading to expulsion from the gastro-intestinal tract. Flavonoids from Chenopodium album L and Mangifera indica L act through the action of phosphodiesterase and Ca2+-ATPase, and flavonoids and tannins have been shown to act synergistically and are complementary to praziquantel. Artemisinins from Artemisia cina O. Berg are known to disrupt mitochondrial ATP production. Terpenoids from Cucurbita moschata L disrupt neurotransmission leading to paralysis as well as disruption of egg hatching. Yeast particle encapsulated terpenes are effective for the control of albendazole-resistant helminths.Item Epidemiology of Trypanosomiasis in Wildlife—Implications for Humans at the Wildlife Interface in Africa(Frontiers in Veterinary Science, 2021) Kasozi, Keneth Iceland; Zirintunda, Gerald; Ssempijja, Fred; Buyinza, Bridget; Matama, Kevin; Nakimbugwe, Helen N.; Onanyang, David; Bogere, Paul; Ochieng, Juma John; Matovu, Wycliff; Nalumenya, David Paul; Batiha, Gaber El-Saber; Osuwat, Lawrence Obado; Omadang, Leonard; Welburn, Susan ChristinaWhile both human and animal trypanosomiasis continue to present as major human and animal public health constraints globally, detailed analyses of trypanosome wildlife reservoir hosts remain sparse. African animal trypanosomiasis (AAT) affects both livestock and wildlife carrying a significant risk of spillover and cross-transmission of species and strains between populations. Increased human activity together with pressure on land resources is increasing wildlife–livestock–human infections. Increasing proximity between human settlements and grazing lands to wildlife reserves and game parks only serves to exacerbate zoonotic risk. Communities living and maintaining livestock on the fringes of wildlife-rich ecosystems require to have in place methods of vector control for prevention of AAT transmission and for the treatment of their livestock. Major Trypanosoma spp. include Trypanosoma brucei rhodesiense, Trypanosoma brucei gambiense, and Trypanosoma cruzi, pathogenic for humans, and Trypanosoma vivax, Trypanosoma congolense, Trypanosoma evansi, Trypanosoma brucei brucei, Trypanosoma dionisii, Trypanosoma thomasbancrofti, Trypanosma elephantis, Trypanosoma vegrandis, Trypanosoma copemani, Trypanosoma irwini, Trypanosoma copemani, Trypanosoma gilletti, Trypanosoma theileri, Trypanosoma godfreyi, Trypansoma simiae, and Trypanosoma (Megatrypanum) pestanai. Wildlife hosts for the trypansomatidae include subfamilies of Bovinae, Suidae, Pantherinae, Equidae, Alcephinae, Cercopithecinae, Crocodilinae, Pteropodidae, Peramelidae, Sigmodontidae, and Meliphagidae. Wildlife species are generally considered tolerant to trypanosome infection following centuries of coexistence of vectors and wildlife hosts. Tolerance is influenced by age, sex, species, and physiological condition and parasite challenge. Cyclic transmission through Glossina species occurs for T. congolense, T. simiae, T. vivax, T. brucei, and T. b. rhodesiense, T. b. gambiense, and within Reduviid bugs for T. cruzi. T. evansi is mechanically transmitted, and T. vixax is also commonly transmitted by biting flies including tsetse. Wildlife animal species serve as long-term reservoirs of infection, but the delicate acquired balance between trypanotolerance and trypanosome challenge can be disrupted by an increase in challenge and/or the introduction of new more virulent species into the ecosystem. There is a need to protect wildlife, animal, and human populations from the infectious consequences of encroachment to preserve and protect these populations. In this review, we explore the ecology and epidemiology of Trypanosoma spp. in wildlife.Item Investigating Vegetable Contamination in Indigent Communities by Heavy Metals: A Case of Food Safety in Bushenyi, Uganda(Environmental Pollutants and Bioavailability, 2021) Otim, Eric Oloya; Kasozi, Keneth Iceland; Zirintunda, Gerald; Matama, Kevin; Otim, OchanFood contamination by heavy metals is a health burden in sub-Saharan Africa. Here, we illustrate this burden by quantifying levels of Cd, Cr, Ni, Co, Pb, Fe, Cu and Zn in vegetables from Bushenyi District (Uganda). Results show that cabbage, scarlet eggplant, tomato and amaranth sold in Bushenyi, Ishaka, Kashenyi, Kizinda and Nyakabirizi open markets contain high levels of Zn and Fe. The uptake of metals overall appeared to be species-specific. Amaranth, for example, had more metals than scarlet eggplant, which in turn had more metals than tomato or cabbage. Within a species, cabbage from Ishaka and Kashenyi presented a combinatorial set of characteristics quite distinct from cabbage from other areas. Such differences arose perhaps from differential capacities to uptake/retain metals from soil or atmospheric particulates. More studies are needed to pinpoint sources of vegetable contamination in Bushenyi. Perhaps then remedial measures can be proposed.Item Misconceptions on COVID-19 Risk Among Ugandan Men: Results From a Rapid Exploratory Survey, April 2020(Frontiers in public health, 2020) Kasozi, Keneth Iceland; MacLeod, Ewan; Ssempijja, Fred; Mahero, Michael W.; Matama, Kevin; Musoke, Grace Henry; Ssebuufu, Robinson; Wakoko-Studstil, Florence; Echoru, Isaac; Mujinya, Regan; Nambuya, Grace; Zirintunda, Gerald; Welbur, Susan ChristinaTransmission of COVID-19 in developing countries is expected to surpass that in developed countries; however, information on community perceptions of this new disease is scarce. The aim of the study was to identify possible misconceptions among males and females toward COVID-19 in Uganda using a rapid online survey distributed via social media. A cross-sectional survey carried out in early April 2020 was conducted with 161 Ugandans, who purposively participated in the online questionnaire that assessed understandings of COVID-19 risk and infection. Sixty-four percent of respondents were male and 36% were female. We found significant divergences of opinion on gendered susceptibility to COVID-19. Most female respondents considered infection risk, symptoms, severe signs, and death to be equally distributed between genders. In contrast, male respondents believed they were more at risk of infection, severe symptoms, severe signs, and death (52.7 vs. 30.6%, RR = 1.79, 95% CI: 1.14–2.8). Most women did not share this perception and disagreed that males were at higher risk of infection (by a factor of three), symptoms (79% disagree), severe signs (71%, disagree), and death (70.2% disagree). Overall, most respondents considered children less vulnerable (OR = 1.12, 95% CI: 0.55–2.2) to COVID-19 than adults, that children present with less symptoms (OR = 1.57, 95% CI: 0.77–3.19), and that there would be less mortality in children (OR = 0.92, 95% CI: 0.41–1.88). Of female respondents, 76.4% considered mortality from COVID-19 to be different between the young and the elderly (RR = 1.7, 95% CI: 1.01–2.92) and 92.7% believed young adults would show fewer signs than the elderly, and 71.4% agreed that elderly COVID-19 patients would show more severe signs than the young (OR = 2.2, 95% CI: 1.4, 4.8). While respondents considered that all races were susceptible to the signs and symptoms of infection as well as death from COVID-19, they considered mortality would be highest among white people from Europe and the USA. Some respondents (mostly male 33/102, 32.4%) considered COVID-19 to be a “disease of whites” (30.2%).The WHO has identified women and children in rural communities as vulnerable persons who should be given more attention in the COVID-19 national response programs across Africa; however, our study has found that men in Uganda perceive themselves to be at greater risk and that these contradictory perceptions (including the association of COVID-19 with “the white” race) suggest an important discrepancy in the communication of who is most vulnerable and why. Further research is urgently needed to validate and expand the results of this small exploratory study.Item Molecular Epidemiology of Anaplasmosis in Small Ruminants along a Human-Livestock-Wildlife Interface in Uganda(Heliyon, 2021) Kasozi, Keneth Iceland; Welburn, Susan Christina; Nalumenya, David Paul; Namayanja, Monica; Matama, Kevin; Zalwango, Kelly Katenta; Matovu, Wycliff; Zirintunda, Gerald; Ekou, Justine; Kembabazi, Stellamaris; Mugasa, Claire Mack; Kitibwa, Annah; Tayebwa, Dickson Stuart; Musinguzi, Simon Peter; Mahero, Michael; Ssengendo, Ibrahim; Nanteza, Anne; Matovu, Enock; MacLeod, Ewan ThomasInformation as regards the epidemiology of the Anaplasmataceae in small ruminants in several low- and middle-income countries is scarce. In this study a total of 712 DNA samples collected from small ruminants were analyzed for Anaplasmataceae and Anaplasma ovis using the 16S rRNA and MSP4 genes respectively. Infection risk was assessed by location, sex and age of the animals and qGIS® was used to construct spatial maps. The prevalence of Anaplasmataceae spp was 89.1% (95% CI: 77.5–95.9) and 79.1% (95% CI: 75.9–82.1) in ovines and caprines respectively (RR = 1.1, 95% CI: 1.0–1.3); higher than those previously reported in other eastern African countries. The prevalence of A. ovis was 26.1% and 25.4% for both ovines and caprines respectively with ovines showing significantly higher levels of infection than caprines (P < 0.05). The risk of Anaplasma ovis infections was not affected by age (OR = 1.2, 95% CI: 0.9–1.7) or sex (OR = 1.1, 95% CI: 0.6–2.0). Small ruminants located at the forest edge (<0.3 km) showed higher A. ovis prevalence than those found inland with infections present in the midland regions associated with increased agricultural activity. Anaplasma ovis remains a major challenge for small ruminant husbandry in Uganda and infections are under-reported. Policy efforts to prioritize management of Anaplasmataceae for small ruminant health would promote livestock productivity in vulnerable communities, improving livelihoods and ecosystem health.Item Pandemic Panic and Anxiety in Developing Countries. Embracing One Health Offers Practical Strategies in Management of COVID-19 for Africa(The Pan African Medical Journal, 2020) Kasozi, Keneth Iceland; Mujinya, Regan; Bogere, Paul; Ekou, Justine; Zirintunda, Gerald; Ahimbisibwe, Salaviriuse; Ninsiima, Herbert IzoThe coronavirus, COVID-19 outbreak has now affected over 60% of African countries in less than two months [1], gaining a foothold through major economic and transport hubs on the African continent including Egypt, Algeria, Nigeria, South Africa and Kenya. Travel restrictions imposed against citizens from countries with major outbreaks including China, USA and those in Europe were too late [2,3]. African Union member states as of early April 2020 are reporting 6,470 cases and 241 deaths from COVID-19 reporting growth as “close to exponential”.