Browsing by Author "Zezi, Abdulkadir U."

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    Anti-nociceptive and anti-inflammatory activities of methanol root extract of Andropogon gayanus Kunth (Poaceae) in rodents
    (African Journal of Pharmacology and Therapeutics, 2020) Zandam, Suleiman U.; Nazifi, Abdullahi B.; Odoma, Saidi; Zezi, Abdulkadir U.; Danjuma, Nuhu M.
    Andropogon gayanus is widely used in traditional medicine for various ailments such as postpartum pain, bronchitis and oedema. Objective: This study evaluated the anti-nociceptive and anti-inflammatory activities of methanol root extract of A. gayanus in experimental rodents. Methods: Phytochemical screening tests and acute toxicity studies were carried out. Analgesic activity using acetic acid-induced writhing response and hot plate test in mice, formalin-induced pain and carrageenan-induced paw oedema in rats were evaluated at doses of 250, 500 and 1000 mg/kg of the extract. Results: Oral median lethal dose was >5000 mg/kg in both mice and rats. The extract significantly (p<0.01) decreased the number of writhing movements at all tested doses. It also significantly (p<0.05) increased the mean reaction times. A significant (p<0.05) decrease in mean pain scores was also observed in both phases of the formalin test at 1000 mg/kg. The extract at 1000 mg/kg significantly (p<0.05) reduced the oedema at the 1st hour, while at the 5th hour, all doses tested significantly reduced the oedema. Conclusion: The methanol root extract of Andropogon gayanus possesses antinociceptive and anti-inflammatory activitiesAndropogon gayanus is widely used in traditional medicine for various ailments such as postpartum pain, bronchitis and oedema. Objective: This study evaluated the anti-nociceptive and anti-inflammatory activities of methanol root extract of A. gayanus in experimental rodents. Methods: Phytochemical screening tests and acute toxicity studies were carried out. Analgesic activity using acetic acid-induced writhing response and hot plate test in mice, formalin-induced pain and carrageenan-induced paw oedema in rats were evaluated at doses of 250, 500 and 1000 mg/kg of the extract. Results: Oral median lethal dose was >5000 mg/kg in both mice and rats. The extract significantly (p<0.01) decreased the number of writhing movements at all tested doses. It also significantly (p<0.05) increased the mean reaction times. A significant (p<0.05) decrease in mean pain scores was also observed in both phases of the formalin test at 1000 mg/kg. The extract at 1000 mg/kg significantly (p<0.05) reduced the oedema at the 1st hour, while at the 5th hour, all doses tested significantly reduced the oedema. Conclusion: The methanol root extract of Andropogon gayanus possesses antinociceptive and anti-inflammatory activities. Keywords:
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    Anti-nociceptive, anti-inflammatory and possible mechanism of antinociceptive action of methanol leaf extract of Nymphaea lotus Linn (Nymphaeceae)
    (Journal of Traditional and Complementary Medicine, 2021) Rege, Musa G.; Ayanwuyi, Lydia O.; Zezi, Abdulkadir U.; Odoma, Saidi
    The leaf of Nymphaea lotus has been used traditionally for the management of pain and inflammatory diseases. The methanol leaf extract of Nymphaea lotus (NLE) was evaluated for possible anti-nociceptive and anti-inflammatory activities in rats and mice (at the doses of 250, 500 and 1,000 mg/kg) to investigate the existence of scientific basis for the folkloric use of the plant. The standard drugs used were piroxicam (10 mg/kg) and morphine (10 mg/kg). The possible pharmacological mechanism involved in the anti-nociceptive activity was also investigated. The acute toxicity was determined in mice and rats using method of Lorke. The anti-nociceptive activity was evaluated using acetic acid-induced writhing and hot plate tests in mice, while the anti-inflammatory activity was evaluated using carrageenaninduced hind paw edema model in rats. The oral median lethal dose of NLE was found to be greater than 5,000 mg/kg in rats and mice. NLE demonstrated significant and dose-dependent protection against acetic acid induced writhes and increased the reaction time of mice in hot plate test. Pretreatment of the animals with naloxone (2 mg/kg) significantly (p < 0.05) attenuated the anti-nociception elicited by both NLE and morphine. NLE at the doses of 250 and 1,000 mg/kg significantly (p < 0.05) decreased rat paw edema at the 2nd hour in the carrageenan-induced paw edema test. The result of the study revealed that Nymphaea lotus possesses anti-nociceptive activities which may be mediated via the opioidergic system as well as mild anti-inflammatory activities thus providing scientific basis for the use of the plant in the management of pain and inflammatory diseases.
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    Sub-Acute Toxicological Evaluation of Methanol Leaf Extract of Nymphaea lotus Linn (Nymphaeceae) in Wistar Rats
    (Tropical Journal of Natural Product Research, 2023) Rege, Musa G.; Ayanwuyi, Lydia O.; Zezi, Abdulkadir U.; Odoma, Saidi; Bisalla, Muhammed
    Nymphaea lotus has been used for centuries as an astringent, aphrodisiac, sedative, analgesic, anti-inflammatory, and for the treatment of infectious diseases. This study is aimed at evaluating the subacute toxicity profile of methanol leaf extract of Nymphaea lotus in Wistar rats. Rats were administered the crude methanol leaf extract orally for 28 days at 250, 500, and 1,000 mg/kg. Weekly body weight, food, and water intake were recorded. On the 29th day, the rats were sacrificed, and their hematological and biochemical parameters were assessed, as well as histological examination of the kidney, liver, stomach, and intestine. The extract had no effect on the body weights, relative organ weights, or food and water intakes of the animals. It had no impact on hematological markers at the tested doses (hemoglobin, packed cell volume, red blood cell, white blood cell, mean corpuscular hemoglobin, mean corpuscular volume, and platelets concentration), except for alkaline phosphate, which was significantly (p≤0.01) greater at the 500 mg/kg. There was no significant effect on liver function parameters evaluated (total bilirubin, direct bilirubin, alanine aminotransferase, aminotransferase, and aspartate). Urea, creatinine and chloride levels were significantly elevated (p≤0.01 and p≤0.05) at 250 and 500 mg/kg doses, but not at the 1,000 mg/kg dose. Histopathological evaluation of the liver, kidneys, stomach, and intestine revealed no notable histological abnormalities. Based on the results, the methanol leaf extract of Nymphaea lotus appears to be generally safe when taken orally at these doses.