Browsing by Author "Wagoire, W.W."

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    Implications of Black Coffee Twig Borer on cocoa in Uganda
    (African Journals Online (AJOL), 2014) Kagezi, G.H.; Kucel, P.; Egonyu, J.P.; Ahumuza, G.; Nakibuule, L.; Kobusinge, J.; Wagoire, W.W.
    Cocoa (Theobroma cacao L.) is one of Uganda’s major cash crops. It is grown by 15,000-18,000 smallholder households on an estimated 20,000 hectares. Cocoa contributes about US$65 million annually to the country’s foreign exchange earnings. On account of its perennial nature and robust vegetative growth, cocoa harbors a wide range of insect pests which affect its production. Here, we report for the first time an outbreak of the Black Coffee Twig Borer (BCTB), Xylosandrus compactus (Eichhoff), a new pest on cocoa in Uganda. To determine its spread and impact, we surveyed 20 households in Bundibugyo, Kibaale and Hoima districts in January 2014. On each field, 10 cocoa trees were examined for BCTB infestation along a transect. Overall, more than half of the cocoa plantations, 13% of trees and 3.8% of primary branches were infested. At district level, Kibaale had the highest proportions of infested fields (100%), trees (30%) and primary branches (8.5%). The seriousness of BCTB prevalence is likely to complicate the current BCTB spray programme on coffee in the country.
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    Using Translation Elongation Factor Gene to Specifically Detect and Diagnose Fusarium xylaroides, a Causative Agent of Coffee Wilt Disease in Ethiopia, East and Central Africa
    (J Plant Pathol Microbiol, 2018) Olal, S.; Olango, N.; Kiggundu, A.; Ochwo, S.; Adriko, J.; Nanteza, A.; Matovu, E.; Lubega, G.W.; Kagezi, G.; Hakiza, G.J.; Wagoire, W.W.; Opiyo, S.O.
    The present study presents the first report on the application of DNA-based polymerase chain reaction (PCR) for the specific detection and diagnosis of F usarium xylarioides (anamorph: G ibberrela xylarioides). Fusarium xylarioides is the causative agent of Coffee wilt disease (Tracheomycosis), and the disease is the most important economic constraint in Robusta coffee production in Uganda. The pathogen has two races, one pathogenic to Robusta coffee and the other to Arabica coffee, and not vice versa. Its laboratory diagnosis has been mainly based on microscopy, which is slow, has poor discriminative power, requires high expertise, only applicable on host plants with symptoms, and has since failed to detect the pathogen from the soil. Translation Elongation factor-1α (TEF-1α) gene from a F. xylarioides isolated from infected Robusta coffee plant was amplified by Fusarium genus specific primer then the PCR product sequenced. The sequence data was then used to design the specific primer. The primer-BLAST product was found to match only F. xylarioides sequences comprising 75% of the race pathogenic to Robusta and 25% to Arabica coffee. In vitro test by PCR showed the primer to be specific to only F. xylarioides amplifying a 284bp product and was able to differentiate F. xylarioides from all closely related species of Fusarium and other plant pathogens tested. More so it was able to amplify DNA from all the F. xylarioides isolates from different regions of Uganda, and amplified DNA concentrations as minute as 0.78 ng/µL.
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    Using Translation Elongation Factor Gene to Specifically Detect and Diagnose Fusarium xylaroides, a Causative Agent of Coffee Wilt Disease in Ethiopia, East and Central Africa
    (J Plant Pathol Microbiol, 2018) Olal, S.; Olango, N.; Kiggundu, A.; Nanteza, A.; Matovu, E.; Wagoire, W.W.
    The present study presents the first report on the application of DNA-based polymerase chain reaction (PCR) for the specific detection and diagnosis of F usarium xylarioides (anamorph: G ibberrela xylarioides). Fusarium xylarioides is the causative agent of Coffee wilt disease (Tracheomycosis), and the disease is the most important economic constraint in Robusta coffee production in Uganda. The pathogen has two races, one pathogenic to Robusta coffee and the other to Arabica coffee, and not vice versa. Its laboratory diagnosis has been mainly based on microscopy, which is slow, has poor discriminative power, requires high expertise, only applicable on host plants with symptoms, and has since failed to detect the pathogen from the soil. Translation Elongation factor-1α (TEF-1α) gene from a F. xylarioides isolated from infected Robusta coffee plant was amplified by Fusarium genus specific primer then the PCR product sequenced. The sequence data was then used to design the specific primer. The primer-BLAST product was found to match only F. xylarioides sequences comprising 75% of the race pathogenic to Robusta and 25% to Arabica coffee. In vitro test by PCR showed the primer to be specific to only F. xylarioides amplifying a 284bp product and was able to differentiate F. xylarioides from all closely related species of Fusarium and other plant pathogens tested. More so it was able to amplify DNA from all the F. xylarioides isolates from different regions of Uganda, and amplified DNA concentrations as minute as 0.78 ng/µL.