Browsing by Author "Tumuhairwe, John B."

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    Assessing Ambient And Internal Environmental Conditions Of Pit Latrines In Urban Slums Of Kampala, Uganda: Effect On Performance
    (Journal of Water, Sanitation and Hygiene for Development, 2017) Nakagiri, Anne; Niwagaba, Charles B.; Nyenje, Philip M.; Kulabako, Robinah K.; Tumuhairwe, John B.; Kansiime, Frank
    There is increasing interest to improve the functionality and performance of pit latrines in low income urban areas. This study aimed at assessing the ambient and pit environmental conditions and their implications on the performance (smell and fly nuisance) of pit latrines. Forty-two pit latrines were investigated in urban slums of Kampala, Uganda, through field observation and measurements of ambient and pit environmental conditions. The implications were assessed using oxygen-reduction potential (ORP) and its association with smell/insect nuisances. The pit temperature (21 to 30.7 °C), pH (5.0–11.8) and ORP (−247 to 65.9 mV) were consistently, significantly different (p < 0.001) between the surface and 0.5 m depth of pit content. The conditions in most (95%) pit latrines were anoxic (ORP < +50 mV), and mainly within the acid formation range (ORP −199 to −51 mV). Most smelling pit latrines and flies were within the acid formation ORP range, with a significant association (gamma, G = 0.797, p = 0.014) between ORP and smell in clean latrines only. The results suggest that ventilation of pit latrines within urban slums was not sufficient. Additionally, cleanliness, moisture reduction and waste stabilisation could address bad smells in pit latrines, ultimately improving their usage in urban slums.
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    Genotype by environment effects on promiscuous nodulation in soybean (Glycine max L. Merrill)
    (Agriculture & Food Security, 2017) Agoyi, Eric E.; Odong, Thomas L.; Tumuhairwe, John B.; Chigeza, Godfree; Diers, Brian W.; Tukamuhabwa, Phinehas
    Understanding factors influencing the expression of a trait is key in designing a breeding program. Genotype by environment interaction has great influence on most quantitative traits. Promiscuous nodulation is a trait of importance for soybean production in Africa, because of the soil bacteria Bradyrhizobium japonicum not being indigenous in most African soils. Most soybean cultivars require B. japonicum for nodulation leading to the need for seed inoculation before sowing soybean in Africa. Few cultivars have capability to nodulate with Bradyrhizobia spp. that are different from B. japonicum and native in African soils. Such cultivars are termed “promiscuous cultivars.” Field experiments were conducted in six locations in Uganda for two seasons, to investigate the extent of environmental influences on the nodulation ability of promiscuous soybean genotypes. Results: Additive main effect and multiplicative interaction effects showed highly significant environment and genotype by environment (G × E) interaction effects on all nodulation traits. G × E interaction contributed more to the total variation than genotypes. The genotypes Kabanyolo I and WonderSoya were the most stable for nodules’ dry weight (NDW), which is the nodulation trait the most correlated with grain yield. Genotype UG5 was the most stable for nodules’ number (NN), and Nam II for nodules’ effectiveness (NE). The genotype NamSoy 4M had the highest performance for NN, NFW, and NDW, but was less stable. WonderSoya had the highest NE. Genotype and genotype by environment analysis grouped environments into mega-environments (MEs), and four MEs were observed for NDW, with NamSoy 4M the winning genotype in the largest ME, and Kasese B the ideal environment for that nodulation trait. Conclusion: This study provides information that can guide breeding strategies. The low genetic effect that led to high environmental and G × E interaction effects raised the need for multi-environments testing before cultivar selection and recommendation. The study revealed genotypes that are stable and others that are high performing for nodulation traits, and which can be used as parental lines in breeding programs.
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    Mode of inheritance of promiscuous nodulation and combining abilities in soybean genotypes
    (International Journal of Agronomy and Agricultural Research (IJAAR), 2016) Agoyi, Eric E.; Mohammed, Khalid E.; Odong, Thomas L.; Tumuhairwe, John B.; Chigeza, Godfree; Tukamuhabwa, Phinehas
    Knowledge of the mode of inheritance of a trait can be a powerful decision-making tool in a breeding program, as it helps predicting selection gain, defining breeding strategy and choosing parental lines. This study aimed at estimating genetic parameters to infer the mode of inheritance of promiscuous nodulation in soybean. Half diallel crosses were made among nine parental lines. F2 progenies were field evaluated together with parents for nodulation characteristics and grain yield in response to Bradyrhizobium sp. strain USDA 3456. Data on nodule number (NN), percent of effective nodules (NE), fresh and dry weight of nodules (NFW and NDW), and grain yield were subjected to analysis of variance, and progenies’ means regression against parents’ was performed following Griffing’s Method2/Model 1. General and specific combining abilities, broad and narrow sense heritabilities, and Baker’s ratio were estimated. The study showed predominant GCA effect for all measured traits except NE. Broad and narrow sense heritabilities were high for grain yield and NDW, moderate for NN and NFW, and low for NE. Baker’s ratio was high for all measured traits except for NE. Overall, additive gene action was more important for all measured traits, except NE where non-additive gene action was more important. The high to moderate heritabilities for most traits showed that substantial gain can be achieved through selection.
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    Performance Of Pit Latrines In Urban Poor Areas: A Case Of Kampala, Uganda
    (Habitat international, 2015) Nakagiri, Anne; Kulabako, Robinah N.; Nyenje, Philip M.; Tumuhairwe, John B.; Niwagaba, Charles B.; Kansiime, Frank
    In many urban poor areas of Sub-Saharan Africa (SSA), demand for human excreta disposal is met, predominantly by pit latrines. This study aimed at determining the status of pit latrines (design, construction, operation and maintenance) and its influence on latrine performance (filling, smell and insect nuisance). The study was conducted on 130 pit latrines in typical urban poor areas of Kampala, Uganda. Data on design, construction, usage, operation and performance of the pit latrines was collected by interviews, observations and measurements; and analysed by descriptive statistics, bi-variate analysis and logistic regression. Results showed that the level of pit content was predicted by rain or storm water entry, terrain, cleaning before or after use and number of households using the latrine. Smell was predicted by cleanliness, stance length, superstructure material and whether the latrine was private or public. The predictor of presence of flies was the superstructure material. To improve the performance of pit latrines in urban poor areas, researchers and practitioners should develop local latrine design standards (dimensions, construction materials and number of users) and cleaning guidelines for local policy makers to implement.