Browsing by Author "Righi, Simona"

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    B-cell non-Hodgkin lymphomas in Uganda: an immunohistochemical appraisal on tissue microarray
    (Human pathology, 2008) Tumwine, Lynnette K.; Campidelli, Cristina; Righi, Simona; Neda, Sophia; Byarugaba, Wilson; Pileri, Stefano A.
    The most common non-Hodgkin lymphomas in Uganda are neoplasms of B-cell derivation. The field of B-cell lymphoma immunophenotype has rapidly progressed because of the increasing availability of markers applicable to routine sections. Although the latter have allowed the identification of distinctive lymphoma entities in the developed countries, such approach has not yet been used in Uganda. One hundred twenty-nine formalin-fixed, paraffin-embedded tissue samples from the Department of Pathology of Makerere University were used for tissue micro-array (TMA) construction. Four-micrometer-thick sections were cut from TMAs and stained with hematoxylin and eosin and Giemsa. Theywere also used for immunohistochemistry and in situ hybridization.According to morphology and immunohistochemistry, lymphoid neoplasms were classified as Burkitt's lymphoma (BL) (95 cases), diffuse large B-cell lymphoma (19 cases), mantle cell lymphoma (4 cases), and B-cell lymphoblastic lymphoma (1 case). In BL, a homogeneous phenotype (CD10+, Bcl-6+, Bcl-2- , MUM1/IRF4-, and Ki-67 ~100%) and a stable Epstein-Barr virus integration were found. A distinctive and unusual feature was the frequent plasma cellular differentiation, along with the positivity for CD30 and CD138 (recorded in 35 and 43 cases, respectively). According to our findings, most non-Hodgkin B-cell tumors in Uganda are endemic BLs followed by diffuse large B-cell lymphomas. The rest consist of rare but clinically important entities such as mantle cell lymphoma and B-cell lymphoblastic lymphoma. The availability of TMAs and immunohistochemistry has enabled us to precisely categorize tumors that have so far been diagnosed in Uganda as “high-grade/aggressive” lymphomas on the basis of cell morphology alone. © 2008 Elsevier Inc. All rights reserved.
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    Immunohistochemical and other prognostic factors in B cell non Hodgkin lymphoma patients, Kampala, Uganda
    (BMC Clinical Pathology, 2009) Tumwine, Lynnette K.; Agostinelli, Claudio; Campidelli, Cristina; Othieno, Emmanuel; Wabinga, Henry; Righi, Simona; Falini, Brunangelo; Piccaluga, Pier Paolo; Byarugaba, Wilson
    Non Hodgkin lymphomas are the most common lymphomas in Uganda. Recent studies from developed countries have shown differences in survival for the different immunophenotypes. Such studies are lacking in Africa where diagnosis is largely dependent on morphology alone. We report immunohistochemical and other prognostic factors in B cell non Hodgkin lymphoma patients in Kampala, Uganda. Methods: Non Hodgkin lymphoma tissue blocks from the archives of the Department of Pathology, Makerere University College of Health Sciences, Kampala, Uganda, from 1991-2000, were sub typed using haematoxylin and eosin, Giemsa as well as immunohistochemistry. Using tissue micro array, 119 biopsies were subjected to: CD3, CD5, CD10, CD20, CD23, CD30, CD38, CD79a, CD138, Bcl-6, Bcl-2, IRTA-1, MUM1/IRF4, Bcl-1/cyclin D1, TdT, ALKc, and Ki-67/Mib1. Case notes were retrieved for: disease stage, chemotherapy courses received and retrospective follow up was done for survival.