Browsing by Author "RUBAIHAYO, P.R."

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    DYNAMICS OF Cercospora zeina POPULATIONS IN MAIZE-BASED AGRO-ECOLOGIES OF UGANDA
    (African Crop Science Journal, 2015-03-02) OKORI,, P.; RUBAIHAYO, P.R.; ADIPALA, E.; DIXELIUS, C.
    Stability of pathogen populations characterised by slow temporal variation is important for durability of disease management systems in any agroecology. Temporal variation in population structure is attributed to factors related to ecology, biology and life history, and varies among organisms and ecosystems. The objective of this study was to investigate genetic variability of Cercospora zeina (previously called Cercospora zeae-maydis Type II) populations in maize (Zea mays) producing areas under Uganda conditions. Populations of the fungus were analysed for genetic variability using a fluorescent amplified fragment length polymorphism (AFLP) technique. Little or no genetic differentiation (ΦFST 0.05) was detected for populations sampled within the same year, within an agroecology. However, a weak to moderate population structure was detected between populations from different locations, within the same (ΦFST = 0.08) or different agroecologies (ΦFST = 0.09). Pair-wise comparisons using ΦFST gene diversity and genetic distance, showed a reduction in genetic diversity in younger populations, suggestive of minor effects of selection and genetic drift. Overall, the data suggest that during the 3 years of study the impact of selection and genetic drift on C. zeina populations in the two Ugandan agroecologies is slow, but progressive leading to homogenetity with agroecologies and differences between agroecologies.
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    Genetics of resistance to groundnut rosette virus disease.
    (African crop science journal, 2014-02-21) KAYONDO, S.I.; RUBAIHAYO, P.R.; EDEMA, R.; OZIMATI, A.; OKELLO, D.K.
    Groundnut Rosette Virus disease (GRD) has long been regarded a major limiting biotic constraint to groundnut production in Sub-Saharan Africa (SSA). The disease is caused by a complex of three viral components that interact in a synergistic fashion resulting into severe crop losses. A study was conducted to better understand the genetics of inheritance of GRD resistance. Nineteen groundnut genotypes among which twelve F2 families populations arising from a 3x4 North Carolina II mating design, were evaluated for their percentage disease severity (PDS) and incidence (PDI). There was significant genetic variability for resistance to GRD among the materials studied with more significant additive gene action as compared to non additive. However, since specific combining ability effects were not so consistent among the F2 family populations, evaluation and testing of progenies alongside with their parents would be more meaningful and selection in the early generations would be the most effective strategy. Further, narrow sense heritability of 53% suggests that prerformance of groundnut progenies could be partly predicted by both parental and individual cross means.
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    IMPROVEMENT OF RESISTANCE TO FUSARIUM ROOT ROT THROUGH GENE PYRAMIDING IN COMMON BEAN
    (African Crop Science Journal, 2012) OBALA, J.; MUKANKUSI, C.; RUBAIHAYO, P.R.; EDEMA, R.
    Fusarium root rot (FRR), caused by Fusarium solani f.sp. , is one of the most serious root rot diseases of common bean (Phaseolus vulgaris L.) throughout the world. Yield losses of up to 84% have been attributed to the disease. Development and deployment of resistant materials is the most feasible approach to managing the disease. The objective of this study was to estimate the number of pyramided Fusarium root rot resistance genes among the four resistant lines and determine their effectiveness in improving levels of resistance to Fusarium root rot in the susceptible bean cultivars. Crosses among four Fusarium root rot (Fusarium solani f.sp. phaseoli) resistant common bean (Phaseolus vulgaris) were developed. They involved six inbred lines, MLB-48-89A (M48), MLB-49-89A (M49), G2333 (G2) and G685 (G6), and two susceptible cultivars, K20 and Kanyebwa, The resistant lines were used to develop a double cross (DC) population. The DC F1 and each resistant parent were crossed to each of the two susceptible cultivars to form five-parent and single crosses, respectively. Developed populations were subjected to Fusarium solani f. sp. phasoeli isolate-3 under screenhouse conditions. There were segregation ratios of 15:1 (χ2 = 1.89, P = 0.17), 61:3 (χ2 = 0.18, P = 0.67) and 249:7 (χ2 = 1.74, P = 0.19) indicating that two, three and four genes independently condition resistance to F. solani in lines G2 x G6, M49 x M48 and (M49 x M48) x (G2 x G6). A good fit of only four genes in the double cross compared to two in the G2 x G6 , and three genes in the M49 x M48 cross suggests that at least one parent in the G2 x G6 cross have the same or closely linked genes as a parent in the M49 x M48 cross. The F1 means of the five-parent cross (FPC) involving either susceptible parent had lower disease scores, though not significantly (P > 0.05), than the single-crosses from that parent. The F2 of both FPC showed less disease than the single-cross (SC) means (P < 0.05) compared to the single-cross (SC) while the F2 of both FPC had a significant negative deviation (P < 0.05). The F2 frequency distributions also showed that the FPC in both Kanyebwa and K20 populations had higher proportions of resistant plants than any of the single crosses in the respective populations. The superior performance of the FPC over the SC demonstrates that combining resistance genes form different FRR resistance sources can provide a stable source of resistance than using single sources of resistance.