Browsing by Author "Orawu, Martin"

Now showing 1 - 4 of 4
  • Thumbnail Image
    Item
    Establishment of an early selection method (criteria) for breeding in cowpea (Vigna unguiculata)
    (Journal of Plant Breeding and Crop Science, 2020) Oside Millicent, Avosa; Orawu, Martin; Ongom Obia, Patrick; Dramadri Onziga, Isaac; Rutayisire, Amandin; Osundwa, Cynthia; Paul, Gibson; Edema, Richard
    Populations with high genetic variability are targeted by breeders as they create opportunity for selection and genetic improvement. To achieve this, multiple populations are created, but resources are often scarce. This calls for identification of populations with the desired traits at early generation. The study was carried out at MUARIK in seasons 2017A and 2017B on 135 F2 and 40 F3 cowpea populations respectively together with 25 parental lines aimed at: Determining best performing populations for yield, resistance to scab, virus and flower thrip based on usefulness criterion and selection index methods. Usefulness criterion computed for yield identified NE 36 x 2392 as the best population. Usefulness criterion computed for yield and its components identified NE 5 x Sanzi as the best population. WC 48A x 2392 was identified as the best population using selection index values that included resistance to virus, thrips, scab, yield and its component and when only yield and its components were fitted in the model. Variability and high yield performance was maintained in the forty best populations identified and therefore amendable for future improvement. No differences were shown among the methods used for selection hence can be adapted for breeding in cowpea.
  • Thumbnail Image
    Item
    Evaluation of Cowpea Genotypes for Resistance to Fusarium redolens in Uganda
    (American Journal of Plant Sciences, 2017) Wanjala Namasaka, Roy; Tusiime, Geoffrey; Orawu, Martin; Gibson, Paul; Nyiramugisha, Josiane; Edema, Richard
    Fusarium redolens, a virulent fungus which causes damping off, leaf yellowing, wilting and root rots has recently been devastating cowpea fields in Uganda. This study aimed at identifying cowpea genotypes that are resistant to Fusarium redolens. Therefore, ninety cowpea genotypes were evaluated two times against a highly virulent Fusarium redolens (isolate from Zombo in Paidha district) in the screen house in 2016. Genotype effect was highly significant (P < 0.001) for root rot severity. Based on the Index of Susceptibility (IS), three genotypes (Asontem, Dan1 LA and IT89KD-88) remained resistant (IS < 3.5) over the two screening periods, 72 moderately resistant (3.5 ≤ IS < 6.5) and 11 susceptible (IS ≥ 6.5). Resistance was found to be enhanced by presence of lateral roots above or at the ground level. Further results suggested a difference in genetic control of resistance to root rots and seed rots caused by Fusarium redolens . All the released varieties tested (SECOW 1 T, SECOW 2 W, SECOW 3 B, SECOW 4 W and SECOW 5 T) had moderate resistance to Fusarium redolens . Correlation analysis revealed root rot severity was strongly correlated to disease incidence (+0.64, P < 0.001), to proportion of plants with lateral roots (−0.56, P < 0.001), to amount of leaf chlorophyll (−0.53, P < 0.001) and to proportion of plants that died prematurely due to Fusarium redolens infection (+0.45, P < 0.001). No significant correlation was detected between root rot severity and proportion of plants that germinated. The established resistance could be exploited for improvement of farmer preferred cowpea varieties towards Fusarium redolens resistance in Uganda.
  • Thumbnail Image
    Item
    Genetic inheritance of resistance to Fusarium redolens in cowpea
    (Journal of Plant Breeding and Crop Science, 2017) Wanjala, Namasaka Roy; Tusiime, Geoffrey; Orawu, Martin; Paul, Gibson; Agbahoungba, Symphorien; Mahulé Elysé Boris, Alladassi; Edema, Richard
    Fusarium related root rots have been associated with reduced cowpea productivity in Uganda. Sources of genetic resistance to Fusarium redolens which was found to be the most virulent have been identified but the mode of inheritance of the genes conferring the resistance is unknown. This study aims to investigate how the genes for resistance to F. redolens are inherited in cowpea. Four F. redolens root rot resistant cowpea genotypes were crossed with four intermediately resistant and 2 susceptible cowpea genotypes using North Carolina mating design II. The F1 and the parents were evaluated and data were collected on resistance to seed rot, leaf chlorophyll amount, produced lateral roots, response to plant mortality and root rot severity. Results revealed that additive gene effects were significant for all evaluated traits and non-additive genetic effects were significant in resistance to seed rot and chlorophyll amount. General combining ability (GCA) effects showed that the Asontem genotype was a good combiner for increased lateral roots production and resistance to root rot. Degree of dominance estimates revealed that response to plant mortality, root rots and increased lateral root production traits were recessively inherited while seed rot and amount of leaf chlorophyll were dominantly inherited.
  • Thumbnail Image
    Item
    Harnessing genotype-by-environment interaction to determine adaptability of advanced cowpea lines to multiple environments in Uganda
    (Journal of Plant Breeding and Crop Science, 2020) Abiriga, Francis; Ongom, Patrick O.; Rubaihayo, Patrick R.; Edema, Richard; Gibson, Paul T.; Dramadri, Isaac; Orawu, Martin
    This study was conducted to determine the yield stability of advanced cowpea lines in diverse agroecological zones of Uganda in order to facilitate documentation requirements for national performance trials (NPT). Thirty cowpea genotypes were evaluated against six checks in three localities, over three growing seasons, making a total of 9 unique environments. The trials were laid in a 6x6 alpha lattice design with three replications and grain yield was the principal trait measured. Single-site and multilocation data were summarized using analysis of variance. Further analysis of stability was visualized using the genotype and genotype by environment interaction (GGE) biplot and the additive main effect and multiplicative interaction (AMMI) models. ANOVA depicted highly significant differences among the genotypes, locations, seasons and GEI for grain yield. Based on AMMI analysis, environmental effect accounted for the most variation (84.7%) in the phenotype followed by GE (9.45%) and genotypes (4.45%), alluding to the complex inheritance of grain yield in cowpea. The polygon view and the average environment coordination view of the GGE biplot revealed Ayiyi as the wining genotype in the major mega environment and the most stable and high yielding across environments respectively. The genotypes Ayiyi, WC64 and ALEGIxACC2 yielded higher than the checks and were very stable. The other genotypes G36 (WC 36), G3 (ACC12xSECOW3B), G32 (WC16), and G14 (MU9) did not outperform the checks but displayed high yield stability and the mean yields were above the overall average. These genotypes were considered desirable for advancement to National Performance Trial for potential release as new improved cowpea cultivars.