Browsing by Author "Olowo, Samuel"

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    High Expression of WT1 and Low Expression of p53 in Archived Blocks of Children with Wilms Tumor in South Western Uganda
    (Dove, 2024-03) Ssenkumba, Brian; Atwiine, Barnabas; Mitala, Yekosani; Adongo, Janet; Olowo, Samuel; Nabulya, Rita; Diaz Anaya, Amnia; Atwine, Raymond
    Abstract Brian Ssenkumba,1 Barnabas Atwiine,2,3 Yekosani Mitala,1 Janet Adongo,4 Samuel Olowo,4 Rita Nabulya,1 Amnia Diaz Anaya,1 Raymond Atwine1 1Department of Pathology, Mbarara University of Science and Technology, Mbarara, Uganda; 2Department of Paediatrics and Child Health, Mbarara University of Science and Technology, Mbarara, Uganda; 3Department of Paediatrics and Child Health, Mbarara Regional Referral Hospital, Mbarara, Uganda; 4Department of Nursing, Mbarara University of Science and Technology, Mbarara, UgandaCorrespondence: Brian Ssenkumba, Department of Pathology, Mbarara University of Science and Technology, P.O. BOX 1410, Mbarara, Uganda, Tel +256701364029, Email ssenkumbabrianldp15@gmail.comPurpose: The determination of the presence or absence of anaplasia in Wilms tumor is difficult sometimes creating diagnostic errors and is worsened by the use of neoadjuvant chemotherapy, which causes cellular alterations that may mimic anaplasia. This study described the histological features of Wilms tumor and their association with WT1 and p53 expression in archived specimens in South Western Uganda.Patients and Methods: A series of 308 formalin-fixed paraffin-embedded tissue blocks belonging to 85 children were retrospectively recruited in the only public Histopathology laboratory in South Western Uganda. Rabbit monoclonal Anti-Wilms tumor protein antibody [(CAN-R9) IHC-56-2] ab89901 and rabbit monoclonal Anti-p53 antibody [E26] ab32389 were used to assess the expression of WT1 and p53, respectively. The expression of WT1 and p53 were reported as proportions, Chi-square was also performed to assess for associations and statistical significance was considered when the p-value was less than 0.05.Results: The median age was 3.5 with an interquartile range of (2– 6) years. Mixed histology was the most common at 35.29% (95% CI:25.77– 46.14). Anaplasia was present in 5.88% (95% CI:2.44– 13.52) of the specimens. p53 and WT1 expressions were 13.0% (95% CI:7.25– 22.04), and 41.0% (95% CI: 31.11– 52.04), respectively. Conclusion: Mixed-type histology is the most common histologic feature of Wilms tumor with high expression of WT1 and a low expression of p53 implying that these can be used routinely to confirm the diagnosis as well as anaplasia in South Western Uganda. Keywords: children, Wilms tumor, p53, WT1, South Western Uganda
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    Knowledge of diabetic foot complication, self-care beliefs and practices among patients attending a tertiary hospital in Eastern Uganda
    (International Journal of Africa Nursing Sciences, 2022) Olowo, Samuel; Iramiot, Jacob Stanley; Ssenyonga, Lydia V.
    Non-communicable diseases are a stumbling block to global development. Among the diseases, diabetes mellitus in particular, features a major public health problem with increasing prevalence. Foot ulcer ranks first among the complication of diabetes mellitus preceded by cellulitis. This study aimed at determining knowledge about diabetic foot complications, foot care practices, and the beliefs of diabetic patients towards diabetes. Materials and Methods: A cross-sectional survey using a validated structured questionnaire was conducted among 199 Type 2 adult diabetic patients (≥18 years) who visited the hospital clinic and the wards during the study period. Results: More than half that is 118(59.3%) reported not to know that they may develop reduced blood flow in their feet, and 136(68.30%) reported unawareness of smoking as a contributor to reduced blood flow in their feet. Less than half which is 90(45.2%) of the patients reported to dry their feet often after washing, than half that is 96 (48.2%) always inspecting the inside of footwear before putting on, and only 54(27.1 %) of the participants reported never to walk barefooted. The patients’ beliefs on the causes of diabetes included; hereditary, bad dietary habits, inactivity, stress, being fat, punishment from God or gods, and witchcraft. Conclusion: Diabetic patients demonstrated limited knowledge of diabetic foot complications and foot care practices. The patient’s beliefs on the causes of diabetes included; hereditary, bad dietary habits, inactivity, stress, being fat, punishment from God or gods, and witchcraft.