Browsing by Author "Olanya, M."

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    Efficacy of different fungicide spray schedules for control of potato late blight in Southwestern Uganda
    (Crop protection, 2003) Kankwatsa, P.; Hakiza, J.J.; Olanya, M.; Kidenamariam, H.M.; Adipala, E.
    Potato cultivars grown in Uganda have low levels of general resistance to late blight. As such, most commercial potato farmers rely on fungicide applications for control of Phytophthora infestans, the causal agent of late blight. This study investigated the comparative advantages of using different frequencies of application of Ridomil (MZ 58), Ridomil once and Dithane M-45 (Mancozeb, 80% WP) subsequently, and Dithane M-45 for management of potato late blight. The highest marginal benefit was achieved by applying Ridomil once and Dithane M-45 subsequently at intervals of 14 and 21 days. The lowest marginal benefit was with sole Ridomil application at 21 day spray interval. With weekly sprays it was more economical to apply Dithane M-45 than Ridomil first followed by Dithane M-45 subsequently.
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    Genetic Diversity of Phytophthora infestans (Mont.) de Bary in the Eastern and Western Highlands of Uganda
    (Journal of Phytopathology, 2002-10-16) Ochwo, M. K. N.; Adipala, E.; Rubaihayo, P. R.; Olanya, M.
    Eight isolates of Phytophthora infestans were recovered from late blight infected samples collected from the districts of Mbale and Mbarara in the Eastern and Western highlands of Uganda in 2001 and analysed using mitochondrial deoxyribonucleic acid (DNA) haplotype and Amplified Fragment Length Polymorphism (AFLP) markers. Polymerase chain reaction amplification with the P2 primer followed by digestion with MspI yielded a three-fragment pattern characteristic of isolates belonging to the US-1 clonal lineage; the polymorphism was confirmed by DNA sequencing. AFLP analysis yielded 60 markers, analysis of which clustered the Ugandan isolates with reference to US-1 isolates (US930258 and US940501). These results suggest that the examined Ugandan isolates belong to the US-1 clonage lineage
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    Metalaxyl resistance, mating type and pathogenicity of Phytophthora infestans in Uganda
    (Crop Protection, 2001) Mukalazi, J.; Adipala, E.; Sengooba, T.; Hakiza, J.J.; Olanya, M.; Kidanemariam, H.M.
    A total of 81 isolates of Phytophthora infestans (Mont.) de Bary were recovered from late blight infected samples collected from different areas of Uganda in 1998/1999. They were analyzed for their resistance to metalaxyl fungicide, mating types, and cross infection between potato and tomato hosts. Sensitivity to metalaxyl was determined by growing isolates on 10% V8 medium amended with 0, 5 and 100μg/ml metalaxyl. Overall 44.4% of the isolates tested were resistant to metalaxyl, 23.5% were intermediate and 31.2% were sensitive. Mating type was determined on 80 isolates using an A1 (1724) isolate and by growing the same isolates in pure culture (selfing). Fifty percent of the isolates produced oospores by matings and selfings, 22.5% by mating only and 10% by selfing only; 18% did not produce oospores in the two tests. Twenty seven of the P. infestans isolates from potato-infected tomato. The majority of these isolates were highly resistant to metalaxyl and produced oospores by either mating or selfing or both.