Browsing by Author "Okao, Moses"

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    Effect of Mode of Auxin Application on Rooting and Bud Break of Shea Tree (Vitellaria paradoxa) Cuttings
    (American Journal of Plant Sciences, 2016) Okao, Moses; Ogwal, Lawrence; Mutoni, Gloria; Oyuko Alip, Samuel; Okullo, John B. L.; Okia, Clement A.
    Vegetative propagation by stem cutting is an important technique applied for agricultural production where rooting success is one of the major aspects. A study to assess the effects of mode of application of rooting hormones (IBA) on adventitious root formation of V. paradoxa stem cuttings was conducted. Accordingly, four application methods were investigated in a 4 × 3 factorial experiment using a Completely Randomized Design (CRD). The application methods were: 24-hour extended soak, foliar spraying, basal quick dip and delayed IBA application method. Thus, the parameters used to determine rooting success were mean root length and root number. The effect of these application methods on occurrence of bud break was also considered. On the whole, root length was observed to be a function of IBA concentration, whereby root length increased significantly (P ≤ 0.05) with an increment in IBA concentration. Stem cuttings subjected to 24-h extended soak at 100 ppm rooted best (59.5% ± 8.33%), where as foliar sprayed stem cuttings exhibited the worst rooting success (11.9 ± 3.06 - 23.8% ± 4.16%). Bud break appeared to decrease with increasing IBA concentration and delaying IBA application enhanced rooting percentage of the quick dip method by 7.1%, 9.5% and 11.9% at 2500 ppm, 3500 ppm and 4500 ppm, respectively. The extended soak method of IBA application at 80 ppm shows potential for large scale production of V. paradoxa through stem cuttings.
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    Variation in Seedling Germination and Growth in Five Populations Of vitellariaparadoxa C.F. Gaertn.subsp. Nilotica: A Threatened useful fruit Tree Species in Uganda
    (Research Square, 2020) Boaz Odoi, Juventine; Odong, Thomas L.; Akias Okia, Clement; Lomoris Okullo, JohnBosco; Okao, Moses; Kabasindi, Harriet; Muchugi, Alice; Gwali, Samson
    We studied seedlings germination and growth performance in an economically and socially important fruit tree species of Vitellaria paradoxaC.F. Gaertn.subsp. nilotica (Kotschy) in Uganda. The study aimed at determining variability in germination of the five shea tree seed lots and seedlings growth performance based on their growth traits. Five populations were considered from four agroecological zones of Uganda and 180 candidates plus trees (16 ethnovarieties) selected as seed trees based on their traits for faster growth and high oil yield. A total of 1204 biological seeds were collected and sown in a general tree nursery at Ngetta Zonal Agricultural Research and Development Institute (NgeZARDI)during the month of June 2018 in a randomized incomplete block design with three replications. Results: Significant variation (χ = 526; p < 0.01) was observed in seedling phenotypic traits within and between populations. Regression equation for height growth and leaf size index were given asy = 0.3787 + 12.671x and y = 0.6483 + 15.413x respectively. Root collar diameter was more correlated to leaf size index (0.425) than to height growth (0.30). Clustering of shea tree seedlings based on phenotypic growth traits revealed one aggregated cluster indicating that most of the seedlings from the five populations were similar (Jaccard index 0.92, p < 0.01). However, clustering based on SNP markers revealed three different populations. We recorded higher growth (χ = 708; p< 0.001) in Arua shea population (19.69 cm)compared to the population average (19.04 cm). Red seeded; thin pulped and hairy fruited shea tree ethnovarieties assumed faster growth than the rest. Conclusions: The phenotypic analysis of shea tree seedlings revealed some fast-growing genotypes from the five studied populations. Thesegenotypes can be selected for faster growth for shea tree breeding in Uganda.