Browsing by Author "Nantongo, M."
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Item Management Regimes established for REDD+ and their Adaptability to the Institutional and Ecological Conditions: A case of Ongo Community Forest, Masindi District, Uganda.(Noragric Report, 2015) Nabanoga, G.; Namaalwa, J.; Ssenyonjo, E.; Nantongo, M.This report is one of the outputs of the project “Man and forests – an evaluation of management strategies for reduced deforestation” which aimed at evaluating the different management strategies undertaken to obtain reduced deforestation in tropical forests and hence maintain the various ecosystem services delivered. One component of this project focused on characterizing the management regimes established in the REDD+ pilot area and how well the REDD+ regime is adapted to the local institutional and ecological conditions. The site under investigation is a communally owned forest known as Ongo community forest, where the Environmental Conservation Trust of Uganda (ECOTRUST), is piloting REDD+ activities. The investigations entailed discussions with the implementing agent the ECOTRUST, local council leaders, forest management committee members, Masindi district technical staff and the local community members.Item Molecular Characterization and Antibiotic Susceptibility of Edwardsiella tarda isolated from Farmed Nile Tilapia and African Catfish from Wakiso, Uganda(Uganda Journal of Agricultural Sciences, 2019) Nantongo, M.; Mkupasi, E. M.; Byarugaba, D. K.; Wamala, S. P.; Mdegela, R. H.; Walakira, J. K.This study was conducted to isolate and characterize Edwardsiella tarda (E. tarda) and assess its antimicrobial susceptibility. The bacterium was isolated in Wakiso District, Uganda, from symptomatic and asymptomatic Nile tilapia and African catfish raised in earthen ponds, tanks and cages between September 2016 and February 2017. The bacterium was then identified using conventional biochemical tests and API 20E test kits and characterized by sequencing 16S rRNA gene. The antibiotic susceptibility of 16 drugs was established using the Kirby BeurDisc diffusion method. Eight E. tarda isolates were identified using conventional biochemical tests but only one isolate was confirmed to be E. tarda by PCR. Phylogenetic analysis indicated a distant relationship with other 16S rRNA gene sequences retrieved from the GenBank. Six virulence genes (CitC, muk, gadB, katB, esaV, and fimA) that enhance bacterial survival and pathogenesis in the host were detected. The isolate registered low levels of antibiotic resistance as it was resistant only to Oxacillin, Vancomycin and Penicillin, to which it is intrinsically resistant. This implies low antibiotic usage in aquaculture in the area. Despite its low occurrence, presence of virulent genes in E. tarda indicates its potential to affect fish and human health.