Browsing by Author "Nabukenya, Immaculate"

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    Anthelmintic Resistance in Gastrointestinal Nematodes in goats and Evaluation of FAMACHA Diagnostic Marker in uganda
    (Veterinary parasitology, 2014) Nabukenya, Immaculate; Akiiki, Chris Rubaire; Olila, Deogracious; Muhangi, Denis; Höglund, Johan
    Gastrointestinal nematodes (GIN) are a challenge to goat production globally causing reduced growth, morbidity and mortality. We report here results of the first nation-wide anthelmintic resistance (AR) study and validation of assessment of clinical anaemia with FAMACHA eye scores in goats in Uganda. From August to December 2012 the efficacy of albendazole (7.5mg/kg), levamisole (10.5mg/kg) and ivermectin (0.3mg/kg) against strongyle nematodes was tested on 33 goat farms in Soroti, Gulu, Mpigi, Mbarara and Ssembabule districts of Uganda. Altogether 497 goats were subjected to a total of 45 different faecal egg count reduction tests (FECRT), each involving 5–20 goats. On one farm all substances were tested. Faecal and blood samples were collected and FAMACHA eye scores evaluated on the day of treatment and 15 days later. A questionnaire survey was conducted on frequency, type and dose of anthelmintics used, farm size and grazing management system. Examination of infective third stage larvae (L3) from pooled faecal cultures demonstrated Haemonchus to be the predominant genus (>75%). Resistance to at least one anthelmintic group was detected on 61% of the 33 farms and in 49% of the 45 test groups. Prevalence of resistance to ivermectin, levamisole and albendazole was respectively 58%, 52% and 38%. Correlation between pre-treatment packed cell volume determinations and FAMACHA scores (r498=−0.89) was significant. Paddock grazing system (Odds ratio 4.9, 95% CI 1.4–17.3) and large farm size of >40 goats (odds ratio 4.4, 95% CI 1.2–16.1) were significant predictors of AR. In all districts, resistance to all three anthelmintics was higher on large-scale goat farms practising mostly paddock grazing. Interestingly, resistance to albendazole, the most commonly used anthelmintic in Uganda, was lower than that to ivermectin and levamisole. We recommend adaptation of FAMACHA to goats to help restrict anthelmintic treatment to heavily infected individuals. This will limit selection pressure and hence delay development of anthelmintic resistance.
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    Coronavirus disease pandemic response in Uganda: government trust, risk perception and willingness to adhere to public health measures among social media users
    (ResearchGate, 2022) Kiconco, Arthur; Kabanda, Richard; Tugume, Abdulaziz; Mwagale, Ritah; Kananathan, Abirahmi; Bakyaita, Tabley; Nabukenya, Immaculate; Lwenge, Mathias; Kalenzi, Prisca U.
    The Coronavirus Disease (COVID-19) pandemic has greatly affected many nations, and continues to be a global public health challenge. There is progress in responding to the pandemic including universal access to vaccines in most of the developed countries while access to the vaccines in resource limited countries is still limited to only priority groups. Despite the availability of vaccines, promotion of preventive measures through public health risk communication remains essential. Effective risk communication depends on understanding population factors that affect the response. This study assessed government trust, risk perception and willingness to adhere to COVID-19 preventive measures among social media users. Methods: This was an online analytical cross-sectional survey in Uganda. Data collection was conducted from 16-27 October 2020 using an online self-administered questionnaire. Data was analyzed using STATA and generalized linear modelling with modified Poisson regression was conducted to determine association between the variables. Results: Of 1,014 respondents, most 69.3% (703) were males, 77.71% (788) had completed tertiary education and 72.3% (733) were salaried employees. Overall trust in government response to COVID-19 pandemic was 40.1%. Most of the respondents 69.9% (n=1008) believed that COVID-19 is extremely likely to spread across the country. However, over a half 65.4% (n=994) perceived little or no risk of getting infected with COVID-19. More than half 53.55% (1014) were willing to receive vaccination against COVID-19 in case it was available. Factors positively associated with trust in government response to COVID-19 included being a student (APR 1.56, CI: 1.14-2.12, p=0.005), being in the 36-59 (APR 1.36, CI: 1.16-1.60, p<0.001) and 60+ (APR 1.98, CI: 1.40-2.28, p<0.001) age groups. Being male was negatively associated with trust in government (APR 0.84, CI: 0.72-0.92, p=0.04). Risk perception was associated with trust in government (APR 1.37 CI: 1.42-1.65, p=0.001). There was high level of willingness to adhere to COVID-19 preventive measures among respondents in the 36-59 (APR 1.02, CI: 1.0-1.03, p<0.01) and 60+ (APR 1.03; CI: 1.0-1.04, p<0.01) age groups while low level of willingness was associated with little or no trust in government response to COVID-19 (APR 0.97, CI: 0.95-0.98, P<0.01). Conclusions: The overall trust in government’s response was low. Majority of respondents believed that COVID-19 is extremely likely to spread across the country, but over a half perceived a low risk of getting infected with the virus. Respondents with low trust in government were less willing to adhere to COVID-19 preventive measures. Therefore, there should be interventions designed to improve people’s trust in government, as well as focusing public health risk communication to addressing misconceptions and misinformation about COVID-19 and improving risk perception by clarifying the key vulnerable populations.
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    Risk factors, person, place and time characteristics associated with Hepatitis E Virus outbreak in Napak District, Uganda
    (BMC infectious diseases, 2017) Amanya, Geofrey; Kizito, Samuel; Nabukenya, Immaculate; Kalyango, Joan; Atuheire, Collins; Nansumba, Hellen; Akena Abwoye, Stephen; Opio, Denis Nixon; Kibuuka, Edrisa; Karamagi, Charles
    Hepatitis E is self-limiting, but can cause death in most at risk groups like pregnant women and those with preexisting acute liver disease. In developing countries it presents as epidemic, in 2014 Hepatitis E Virus (HEV) outbreak was reported in Napak district Uganda. The role of factors in this setting that might have propagated this HEV epidemic, including host, agent, and environmental characteristics, were still not clear. This study was therefore conducted to investigate the risk factors, person, place and time characteristics, associated with the hepatitis E virus (HEV) epidemic in Napak district. Methods: Review of line lists data for epidemiological description and matched case control study on neighborhood and age in the ratio of 1:2 were used to assess risk factors for HEV outbreak in Napak. Cluster and random sampling were used to obtain a sample size of 332, (111 cases, 221 controls). Possible interaction and confounding was assessed using conditional logistic regression. Results: Over 1359 cases and 30 deaths were reported during 2013/2014 HEV outbreak. The mean age of patients was 29 ± years, 57.9% of cases were females. Overall case Fatality Ratio was 2.2% in general population but 65.2% in pregnant women. More than 94% of the cases were reported in the sub counties of Napak, 5.7% of cases were reported in the outside neighboring districts. The epidemic peaked in January 2014 and gradually subsided by December 2014. Risk factors found to be associated with HEV included drinking untreated water (OR 6.69, 95% CI 3.15–14.16), eating roadside food (OR 6.11, 95% CI 2.85–13.09), reported not cleaning utensils (OR 3.24, 95% CI 1.55–1.76), and being a hunter (OR 1.14, 95% CI 1.03–12.66). Conclusion: The results of this study suggest that the virus is transmitted by the feco-oral route through contaminated water. They also suggest that active surveillance and appropriate measures targeting community and routine individual health actions are important to prevent transmission and decrease the deaths.
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    Transcriptome analysis of peripheral blood of Schistosoma Mansoni Infected Children from the Albert Nile Region in Uganda Reveals Genes Implicated in Fibrosis Pathology.
    (bioRxiv, 2023) Namulondo, Joyce; Nyangiri, Oscar Asanya; Kimuda, Magambo Phillip; Nambala, Peter; Nassuuna, Jacent; Egesa, Moses; Nerima, Barbara; Biryomumaisho, Savino; Nabukenya, Immaculate; Drago, Kato; Tweyongyere, Robert; Matovu, Enock; Mulindwa, Julius; Mugasa, Claire Mack
    Over 290 million people are infected by schistosomes worldwide. Schistosomiasis control efforts focus on mass drug treatment with praziquantel (PZQ), a drug that kills the adult worm of all Schistosoma species. Nonetheless, re-infections have continued to be detected in endemic areas with individuals living in the same area presenting with varying infection intensities. Our objective was to characterize the transcriptome profiles in peripheral blood of children between 10 - 15 years with varying intensities of Schistosoma mansoni infection living along the Albert Nile in Uganda. RNA extracted from peripheral blood collected from 44 S. mansoni infected (34 high and 10 low by circulating anodic antigen [CAA] level) and 20 uninfected children was sequenced using Illumina NovaSeq S4 and the reads aligned to the GRCh38 human genome. Differential gene expression analysis was done using DESeq2 and enriched pathways in differentially expressed genes (DEGs) were identified using REACTOME. Principal component analysis revealed clustering of gene expression by gender when S. mansoni infected children were compared with uninfected children. In addition, we identified 14 DEGs between S. mansoni infected and uninfected individuals, 56 DEGs between children with high infection intensity and uninfected individuals, 33 DEGs between those with high infection intensity and low infection intensity and no DEGs between those with low infection and uninfected individuals. We also observed upregulation and downregulation of some DEGs that are associated with fibrosis and its regulation. These data suggest expression of fibrosis associated genes as well as genes that regulate fibrosis in S. mansoni infection. The relatively few significant DEGS observed in children with schistosomiasis suggests that chronic S. mansoni infection is a stealth infection that does not stimulate a strong immune response.