Browsing by Author "Laudisoit, Anne"

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    Identification of Flea Blood Meals Using Multiplexed Real-Time Polymerase Chain Reaction Targeting Mitochondrial Gene Fragments
    (The American journal of tropical medicine and hygiene, 2009) Woods, Michael E.; Montenieri, John A.; Eisen, Rebecca J.; Zeidner, Nordin S.; Borchert, Jeff N.; Laudisoit, Anne; Babi, Nackson; Atiku, Linda A.; Enscore, Russell E.; Gage, Kenneth L.
    is found in the West Nile region of Uganda and Democratic Republic of the Congo where flea vectors are often found inhabiting homes. We have developed a multiplexed, real-time polymerase chain reaction assay targeting mitochondrial genes that is capable of detecting blood meal sources in fleas collected off-host in East Africa. Laboratory tests showed that the assay is specific for the intended targets and has a detection limit below one picogram of DNA. Testing of wild-caught fleas from the Democratic Republic of Congo suggests that humans are at significant risk from flea-borne disease and implicates domestic animals including cats, chickens, and the black rat as potential sources of human exposure to fleas and flea-borne diseases. Future application of the assay will help us better define the ecology of plague in East Africa to implement effective control measures to combat the spread of disease.