Browsing by Author "Kigozi, Abasi"

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    Effect of Different Organic Substrates on Reproductive Biology, Growth and Offtake of the African Night Crawler Earthworm (Eudrilus eugeniae)
    (Saly Portudal, Senegal, 2018) Kabi, Fred; Kayima, Denis; Kigozi, Abasi; Mpingirika, Eric Zadok; Kayiwa, Ronald; Okello, Dorothy
    Rapid growth and high fecundity of Eudrilus eugeniae makes it a commercial vermicomposting agent. The worm is also a rich protein source (50-70%CP) in livestock diets. The major question, however, is how do we promote earthworm production as a strategy for ecological livestock intensification and integration with crops through earthworm domestication as a source of protein and vermicompost. Reproduction characteristics, growth and offtake of E. eugeniae were studied using four organic substrates including abattoir waste (AW), cattle manure (CM), soya bean crop residue (SBCR) and a mixture of cattle manure and soya bean crop residue (CM+SBCR) aged 15 days. Irrespective of the substrate, length and biomass of earthworms increased at a decreasing rate between the 1st and 8th weeks. Clitellum appearance was initiated at 31.5±2.4, 32.8±3.2, 33.7±3.3 and 35.5±2.4 days for AW, CM,CM+SBCR and BCR, respectively, while cocoon initiation was at 69.0±1.4 (AW), 54.9±2.3 (CM), 51.7±1.7 (CM+SBCR) and 60.0±2.4 (SBCR) days. Organic substrate used affected reproductive biology,
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    Genome-wide diversity and structure variation among lablab [Lablab purpureus (L.) Sweet] accessions and their implication in a Forage breeding program
    (Genetic Resources and Crop Evolution, 2021) Sserumaga, Julius Pyton; Kayondo, Siraj Ismail; Kigozi, Abasi; Kiggundu, Muhammad; Namazzi, Clementine; Walusimbi, Kato; Bugeza, James; Molly, Allen; Mugerwa, Swidiq
    Most orphan crops have not been fully sequenced, hence we rely on genome sequences of related species to align markers to different chromosomes. This hinders their utilisation in plant population improvement programs. Utilising the advances in the science of sequencing technologies, the population structure, relatedness, and genetic diversity among accessions can be assessed quickly for better exploitation in forage breeding programs. Using DArTseq technology, we studied the genetic and structural variation in 65 Lablab purpureus (L.) Sweet conserved gene-bank accessions using 9320 DArTseqbased SNPs and 15,719 SilicoDart markers. These markers had a low discriminating ability with mean polymorphic information content (P.I.C.) of 0.14 with DArTseq-based SNPs and 0.13 with SilicoDart markers. However, the markers had a high mean call rate of 73% with DArTseq-based SNPs and 97% with SilicoDart markers. Analysis of molecular variance revealed a high within populations variance (99.4%), indicating a high gene exchange or low genetic differentiation (PhiPT = 0.0057) among the populations. Structure analysis showed three allelic pools in variable clusters of DK = 3 and 6. Phylogenetic tree of lablab accessions showed three main groups with variable membership coefficients. Most pairs of accessions (40.3%) had genetic distances between 0.10 and 0.15 for SilicoDart markers, while for DArTseq-based SNPs, (46.5%) had genetic distances between 0.20 and 0.25. Phylogenetic clustering and minimum spanning analysis divided the 65 accessions into three groups, irrespective of their origin. For the first time, this study produced high-density markers with good genom coverage. The utilisation of these accessions in a forage program will base on the information from molecular-based grouping. The outcomes uncovered the presence of noteworthy measure of variety in Uganda, CIAT and ILRI accessions, thus demonstrating an opportunity for further marker-trait-association studies.