Browsing by Author "Keith, Charles H."
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Item Micro-Perfusion Flow Cell for Imaging Cultured Cells(Biotechniques, 1999) Agnihotri, Naveen; Kisaalita, William S.; Keith, Charles H.We present a unique design for a flow cell with a small working volume that allows rapid displacement of media viewed under high power and short working distance objectives. The flow cell has a small internal depth (ca. 0.033 cm) and volume (ca. 0.05 mL) and is easy to handle. Made of Delrin®, the flow cell is biologically inert. We have used the flow cell for fluorescence imaging of PC12 cells loaded with tetramethylrhodamine dextran (TMRD) and other dyes.