Browsing by Author "Kaweesi, Tadeo"
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Item Eleven years of breeding efforts to combat cassava brown streak disease(Breeding Science, 2016) Sezi Kawuki, Robert; Kaweesi, Tadeo; Esuma, Williams; Pariyo, Anthony; Kayondo, Ismail Siraj; Ozimati, Alfred; Kyaligonza, Vincent; Abaca, Alex; Orone, Joseph; Tumuhimbise, Robooni; Nuwamanya, Ephraim; Abidrabo, Philip; Amuge, Teddy; Ogwok, Emmanuel; Okao, Geoffrey; Wagaba, Henry; Adiga, Gerald; Alicai, Titus; Omongo, Christopher; Bua, Anton; Ferguson, Morag; Kanju, Edward; Baguma, YonaCassava (Manihot esculenta Crantz) production is currently under threat from cassava brown streak disease (CBSD), a disease that is among the seven most serious obstacles to world’s food security. Three issues are of significance for CBSD. Firstly, the virus associated with CBSD, has co-evolved with cassava outside its center of origin for at least 90 years. Secondly, that for the last 74 years, CBSD was only limited to the low lands. Thirdly, that most research has largely focused on CBSD epidemiology and virus diversity. Accordingly, this paper focuses on CBSD genetics and/or breeding and hence, presents empirical data generated in the past 11 years of cassava breeding in Uganda. Specifically, this paper provides: 1) empirical data on CBSD resistance screening efforts to identify sources of resistance and/or tolerance; 2) an update on CBSD resistance population development comprising of full-sibs, half-sibs and S1 families and their respective field performances; and 3) insights into chromosomal regions and genes involved in CBSD resistance based on genome wide association analysis. It is expected that this information will provide a foundation for harmonizing on-going CBSD breeding efforts and consequently, inform the future breeding interventions aimed at combating CBSD.Item Physicochemical analysis of Ugandan tea (Camellia sinensis) germplasm reveals potential commercial green and black tea varieties(Taylor & Francis, 2023-05) Grace Nalugo, Racheal; Kaweesi, Tadeo; Kawooya, Ronald; Nuwamanya, Ephraim; Mugisa, Charles; Namutebi, Vivian; Tumwine, Venansio; Turyahebwa, Vereriano; Tumuhimbise, RobooniTea (Camellia sinensis L.) is an important beverage consumed worldwide. In Uganda, it is the second-largest and highly priori-tized export crop after coffee and provides the much-needed jobs to more than 800,000 people. Despite its importance in the coun-try, the crop has received very limited research attention for its improvement and optimal utilization. This study was aimed at characterizing existing tea germplasm in Uganda to inform future breeding initiatives for market-preferred tea varieties. Fifty-eight advanced tea clones were randomly selected from the tea germ-plasm conserved at Uganda’s Tea Research Center in Rwebitaba and analyzed in the laboratory for eight physicochemical descrip-tors. Hierarchical cluster analysis performed on the 58 clones using Cluster-R package revealed four main clusters, indicating the exis-tence of variation for physicochemical parameters within tested germplasm. This variation can be exploited to select among and/or improve the studied germplasm genetically for quality. High fer-mentation rate, which is one of the key indicators for good-quality black teas, revealed 15 fast-fermenter tea clones. Clone “UTR12/12” was identified as the most rapid fermenter, fully fermenting within 30 min, which implies a good clone for black tea. The clone also had the highest polyphenol content (26.7%), higher than the high- quality clone “UTR6/8” (control). Other tea clones whose polyphe-nol content was within the range of the control black tea clone were: “UTR144/10” (20.9%) and ‘UTR144/17, (20.66%). The identi-fied promising black tea clones can be advanced to multi-location trials for further evaluation and selection for eventual variety release as commercial black-tea clones.Item Stability of Solanum aethiopicum Shum accessions under varied water deficit stress levels and identification of pertinent breeding traits for resistance to water shortage(Euphytica, 2018) Sseremba, Godfrey; Tongoona, Pangirayi; Yaw Eleblu, John Savior; Danquah, Eric Yirenkyi; Kaweesi, Tadeo; Baguma, Yona; Masanza, Michael; Balyejusa Kizito, ElizabethDrought is a major constraint to productivity of Solanum aethiopicum ‘Shum’ group due to loss in market and nutrient value of stressed plants. This study evaluated S. aethiopicum Shum group accessions to identify genotypes (G) that excel across moisture deficit stress levels (WLs). A split-plot arrangement composed of four WLs and twenty accessions of S. aethiopicum as main plot and subplot factors, respectively, was implemented in a screenhouse, and repeated for two experiments. In each experiment, there was a highly significant effect of at least two WLs on mean performance among at least two accessions for most of the traits at p\0.05. Further, very highly significant WL 9 G interactions were obtained for leaf relative water content (LRWC), leaves per plant (LPP) and plant height (PH), and nonsignificant for leaf blade length and leaf blade width. The order of priority as breeding traits for stability superiority across WLs was suggested as LRWC[PH[LPP. Consequently, based on LRWC, the most superiorly stable accessions were identified as accession 160 followed by accessions 145, 137, 108P and 184G while the least stable ones were identified as accessions 163G, 141, 163 and 108. The broad sense heritability (H2) for each of the three recommended traits for drought resistance breeding was above 0.9 thus supportive for a good response to selection. Drought stress negatively affected the performance of S. aethiopicum Shum group but the exhibited variation allowed for selection of superiorly stable genotypes.