Browsing by Author "Kaaya, Archileo N."
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Item Awareness of mycotoxins and occurrence of aflatoxins in poultry feeds and feed ingredients in selected regions of Uganda(International Journal of Food Contamination, 2020) Nakavuma, Jesca L.; Kirabo, Angella; Bogere, Paul; Nabulime, Margaret M.; Kaaya, Archileo N.; Gnonlonfin, BenoitBackground: Aflatoxins are a category of mycotoxins produced by certain molds naturally occurring as food and feed contaminants with toxic effects to both animals and humans. In Uganda, previous studies on aflatoxins mainly considered human foods, but scarce information exists for animal feeds. The study aimed at establishing the current status of aflatoxins contamination of poultry feeds, level of awareness and the existing technological challenges and innovations to mycotoxin control in Uganda. Method: Mycotoxin awareness, predisposing factors and existing strategies for managing mycotoxin contamination were investigated through focus group discussions and questionnaires with selected processors and farmers. Poultry feed and feed ingredient samples were collected and analyzed for total aflatoxins using VICAM Fluorimeter procedure for foods and animal feeds. Results: Majority of the farmers and processors (> 50%) had limited knowledge about aflatoxins; contamination predisposing factors; dangers to animals and humans; and mitigation strategies. The study further revealed poor feed and feed ingredients handling and storage practices that predispose to mold/aflatoxin contamination. Forty feed samples from feed processing plants had aflatoxins in the range 7.5 ± 0.71 to 393.5 ± 19.09 parts per billion (ppb) with only twelve samples being within the generally acceptable limits of 20 ppb as recommended by the Food and Agricultural Organisation and the United States Federal Department of Agriculture. Additionally, all 27 feed samples obtained from the farmers had aflatoxins in the range of 19.0 ± 1.41 to 188.5 ± 2.12 ppb and were above the acceptable limit. Generally, broiler feeds were the most contaminated with aflatoxins. Of the feed ingredients tested, silver fish (Rastrineobola argentea, locally known as “mukene”) had the least concentration (8.7 ± 3.18 ppb) of aflatoxins while maize bran had the highest level of contamination, 103.3 ± 22.98 ppb.Item Development and Validation of a Competency-Based Education Model Suitable for Undergraduate Human Nutrition and Dietetics Training in Uganda: A Modified Delphi Process(Research square, 2021) Kikomeko, Peterson Kato; Ochola, Sophie; Kaaya, Archileo N.; Ogada, Irene; Birungi, Tracy Lukiya; Nakitto, PeaceCompetency-based education (CBE) equips health professionals with the requisite competencies for health systems performance. This study aimed to develop and validate a CBE model suitable for teaching Human Nutrition/Human Nutrition and Dietetics (HN/HND) at the undergraduate level in Uganda.Item The in vitro Efficacy of Two Microbial Strains and Physicochemical Effects on their Aflatoxin Decontamination in Poultry Feeds(2020) Tebetyo, Zakia; Bogere, Paul; Nabulime, Margaret M.; Kaaya, Archileo N.; Gnonlonfin, Benoit; Ntale, Mohammed; Nakavuma, JescaContamination of animal feeds with aflatoxigenic fungi is a challenge to livestock farmers worldwide. Aflatoxins are very toxic fungal metabolites that are associated with carcinogenic, mutagenic, teratogenic, and estrogenic effects. The toxins affect animal productivity and may lead to deaths, causing enormous economic losses. Aflatoxin decontamination is a challenge to the feed industry, despite the several approaches available. This study investigated the efficacy of two microbial isolates, Bacillus spp (B285) and Yeast strain (Y833), in reducing Aflatoxin concentration in poultry feeds in comparison with a commonly used commercial chemical binder, Bentonite. The influence of the poultry feed matrix, pH, and temperature on the aflatoxin reducing activity by the two microorganisms was also explored. Results: The in vitro studies showed that the two microorganisms and the chemical binder reduced aflatoxins by over 74% of the original concentration. The chemical binder registered the highest reduction at 93.4%; followed by Y833 (83.6%), then the combination of Y833 and B285 (77.9%); and lastly B285 (74.9%). There was no significant (p>0.05) influence of temperature on the toxin reducing capacity of all the agents tested. The pHs 4.5 and 6.5 did not have a significant effect on the performance of both chemical binder and biological agents, however, the former performed better at pH 6.5 with 95% aflatoxin reduction compared to the microorganisms. The aflatoxin reducing activity was lower in presence of feeds compared to that in Phosphate Buffered Saline except for Y833 where no difference was observed. Conclusions: Although the feed components affected the aflatoxin reducing capacity of the test materials, the chemical binder was more effective than the microbial agents. Yeast strain was more effective than the bacterial strain in reducing the aflatoxin levels, however, both are promising strategies for countering the aflatoxin challenges in animal feeds. In response to the advocacy for use of biological control agents, there is need for more investigations to establish the safety of the microorganisms, the mechanism of decontamination and safety of the products; the optimum concentrations that can reduce aflatoxins in feeds to permissible levels and the effect of the toxin contamination levels on microbial efficiency.Item Management of Aflatoxins in Groundnuts: A manual for Farmers, Processors, Traders and Consumers in Uganda(National Agricultural Research Organization, 2010) Okello, David K.; Kaaya, Archileo N.; Bisikwa, Jenipher; Were, Moreen; Oloka, Herbert K.Groundnut (Arachis hypogaea L.), also known as peanut, is the second most important legume after beans (Phaseolus vulgaris L.) grown mainly in Eastern and Northern Uganda but consumed widely throughout the country (Okello et al., 2010). There has been a substantial increase in groundnut production as both a food and cash crop because of increased awareness of their value as a source of protein (23-25% content), fat (40-50%), oil (40-52% content), and 10-20 % carbohydrate depending on the variety (Savage and Keenan, 1994). With the costs of animal protein ever increasing, groundnut is becoming an even more important source of protein. A kilogram of groundnuts is high in food energy and provides approximately the same energy value as 2 kilograms of beef, 4 litres of milk, or 36 medium-size eggs. Groundnut seeds are also a nutritional source of vitamin E, niacin, falacin, calcium, phosphorus, magnesium, zinc, iron, riboflavin, thiamine and potassium. Groundnut is consumed raw, roasted, blanched, as peanut butter, crushed and mixed with traditional dishes as a sauce or as binyebwa, a cooked paste. These qualities make groundnut an important nutritional supplement to mainly cereal diets of maize, millet and sorghum of many Ugandans.Item Oil Content And Physicochemical Characteristics Of Some Wild Oilseed Plants From Kivu Region Eastern Democratic Republic Of Congo(African Journal of Biotechnology, 2011) Minzangi, Kazadi; Kaaya, Archileo N.; Kansiime, Frank; Tabuti, John R.S.; Samvura, BashwiraSeeds were collected from Carapa grandiflora, Carapa procera, Cardiospermum halicacabum, Maesopsis eminii, Millettia dura, Myrianthus arboreus, Myrianthus holstii, Pentaclethra macrophylla, Podocarpus usambarensis, Tephrosia vogelii and Treculia africana from Kahuzi-Biega National Park and the surrounding areas in D.R. Congo. Oils were extracted using ethyl ether in Soxhlet extractor. Physicochemical characteristics were determined using the methods of the American Oil Chemists Society. The seed oil content obtained ranged from 17.2 to 64.4%; the highest was obtained from P. usambarensis and the lowest from T. vogelii. The oil specific gravity varied from 0.8050 to 0.9854; the oils melting point ranged from -12 to 32°C; the oil saponification values from 182.5 to 260.9 mg KOH/g; the oil acidity index from 1.74 to 5.31 mg KOH/g and the unsaponifiable matter from 0.54 to 2.25%. The plant seed oils content reported in this study are comparatively higher than some food crop plants such as soybean and olive. Five of these oils have oil melting range as that of edible oils. C. grandiflora, C. halicacabum, M. eminii and the two species of Myrianthus are in the range of common cooking oils by their specific gravity values. P. usambarensis seed oil with its relatively high unsaponifiable matter content can have efficacy as cosmetic.Item Stakeholders’ perceptions of the nutrition and dietetics needs and the requisite professional competencies in Uganda: a cross-sectional mixed methods study(BMC Health Services Research, 2021) Kikomeko, Peterson Kato; Ochola, Sophie; Kaaya, Archileo N.; Ogada, Irene; Birungi, Tracy Lukiya; Nakitto, PeaceEffective implementation of nutrition and dietetics interventions necessitates professionals in these fields to possess the requisite competencies for health systems performance. This study explored the stakeholders’ perceptions of the community nutrition and dietetics needs, the nature of work done by graduates of the Bachelor’s degree in Human Nutrition/Human Nutrition and Dietetics (HN/HND), and the competencies required of Nutrition and Dietetics professionals in Uganda.