Browsing by Author "Hakiza, G.J."

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    Ecological factors influencing incidence and severity of Coffee Leaf Rust and Coffee Berry Disease in major Arabica coffee growing districts of Uganda
    (Uganda Journal of Agricultural Sciences, 2013) Matovu, R.J.; Kangire, A.; Phiri, N.A.; Hakiza, G.J.; Kagezi, G.H.; Musoli, P.C.
    Coffee Leaf Rust and Coffee Berry Disease are the most devastating diseases of Arabica coffee in Africa. The importance of coffee in economies of many African countries like Uganda, presents urgent need for cost-effective disease control strategies. In this study, 192 coffee farms were surveyed and their corresponding incidence and severity recorded. Nebbi district had the highest CLR incidence (90.2%) and severity (2.2%) followed by Sironko (67.9% and 1.9%) and least in Kapchorwa (20.4% and 1.3%) respectively. CBD incidence was highest in Kapchorwa (6.0%) followed by Nebbi (1.7%).There was no CBD incidence observed in Sironko. There was a significant (pd”0.05) interaction between altitude and disease severity. Thin and medium shade intensity had highest CLR incidence followed by thick and no-shade levels. CLR was highest in farms under mono-shade followed by farms under mixed-shade and least in open-farms. CLR severity was found to be highest at very steep slopes and medium slopes and least on gentle slopes. In conclusion, CLR was present in all surveyed districts while CBDoccurred in Kapchorwa and Nebbi districts at intensity levels enough to trigger economic losses.
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    Using Translation Elongation Factor Gene to Specifically Detect and Diagnose Fusarium xylaroides, a Causative Agent of Coffee Wilt Disease in Ethiopia, East and Central Africa
    (J Plant Pathol Microbiol, 2018) Olal, S.; Olango, N.; Kiggundu, A.; Ochwo, S.; Adriko, J.; Nanteza, A.; Matovu, E.; Lubega, G.W.; Kagezi, G.; Hakiza, G.J.; Wagoire, W.W.; Opiyo, S.O.
    The present study presents the first report on the application of DNA-based polymerase chain reaction (PCR) for the specific detection and diagnosis of F usarium xylarioides (anamorph: G ibberrela xylarioides). Fusarium xylarioides is the causative agent of Coffee wilt disease (Tracheomycosis), and the disease is the most important economic constraint in Robusta coffee production in Uganda. The pathogen has two races, one pathogenic to Robusta coffee and the other to Arabica coffee, and not vice versa. Its laboratory diagnosis has been mainly based on microscopy, which is slow, has poor discriminative power, requires high expertise, only applicable on host plants with symptoms, and has since failed to detect the pathogen from the soil. Translation Elongation factor-1α (TEF-1α) gene from a F. xylarioides isolated from infected Robusta coffee plant was amplified by Fusarium genus specific primer then the PCR product sequenced. The sequence data was then used to design the specific primer. The primer-BLAST product was found to match only F. xylarioides sequences comprising 75% of the race pathogenic to Robusta and 25% to Arabica coffee. In vitro test by PCR showed the primer to be specific to only F. xylarioides amplifying a 284bp product and was able to differentiate F. xylarioides from all closely related species of Fusarium and other plant pathogens tested. More so it was able to amplify DNA from all the F. xylarioides isolates from different regions of Uganda, and amplified DNA concentrations as minute as 0.78 ng/µL.