Browsing by Author "Atuhaire, Andrew M."

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    Optimizing bio-physical conditions and pre-treatment options for breaking lignin barrier of maize stover feed using white rot fungi
    (Animal Nutrition, 2016) Atuhaire, Andrew M.; Kabi, Fred; Okello, Samuel; Mugerwa, Swidiq; Ebong, Cyprian
    The greatest limitation to utilization of maize stover by ruminants as a feed is the high concentration of lignin, which limits fibre digestibility.However, ruminants can effectively utilize maize stover if its nutritive value is improved using white rot fungal species. This study was designed to determine optimal biophysical conditions for mycelial growth and select the most ideal fungal species and pre-treatment options for improving nutritive value of maize stover. Four popular edible Pleurotus fungal species (viz. Pleurotus florida, Pleurotus ostreatus, Pleurotus sajor caju and Pleurotus pulmonarius) were subjected to varying temperatures, pH levels, hydrogen peroxide (H2O2) concentration and illumination to establish the extent ofmycelial growth rate. Inclusion of H2O2was used to determine optimal levels for preservation and prevention of contamination from other indigenous microbiota. Effects of pre-treatment options on chemical composition and nutritive value of maize stover were also examined. Mycelial growth rate of Pleurotus species on potato dextrose agar (PDA) varied (P < 0.05) with temperature, pH level and H2O2 concentration following a quadratic trend. Optimal temperature, pH and H2O2 concentration for mycelial growth on PDA were 25 C, 5 and 0.01 mL/L, respectively. Under the different bio-physical conditions, P. sajor caju had the highest mycelia density and growth rate. Chemical composition of solid-state fermented maize stover differed (P < 0.05) among the Pleurotus species. Maize stover fermented with P. sajor caju had the highest crude protein (CP) of 86.6 g/kg DM, in-vitro dry matter digestibility (IVDMD) of 731 g/ kg DM, in-vitro organic matter digestibility (IVOMD) of 670.4 g/kg DM and metabolizable energy (ME) of 10.0 MJ/kg DM but with the lowest lignin (sa) of 50 g/kg DM. At 25 C, P. sajor caju had the highestmycelial growth rate on PDA and highest lignin (sa) breakdown in the maize stover substrate. It was, therefore, selected as the most ideal fungal species for improving nutritive value of maize stover. Pre-treatment of maize stover with Lactobacillus plantarum and molasses under anaerobic condition for 7 days before inoculation with P. sajor caju resulted into a substratewith the highest (P < 0.05) CP (96.6 g/kg DM), IVDMD (752.3 g/kg DM), IVOMD (687.2 g/kg DM) and ME (10.2 MJ/kg DM). However, neutral detergent fiber exclusive of residual ash (NDFom) and lignin (sa) fractions decreased (P < 0.05) as a result of subjecting maize stover to pre-treatment with L. plantarum and molasses prior to fermentation with P. sajor caju.
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    Prioritization of Agro-industrial By-products for Improved Productivity on Smallholder Dairy Farms in the Lake Victoria Crescent, Uganda
    (Frontiers in Science, 2014) Atuhaire, Andrew M.; Mugerwa, Swidiq; Okello, Samuel; Lapenga, Kenneth; Kabi, Fred; Lukwago, George
    Livestock feed fluctuations in quality and quantity is a major constraint to dairy cattle production in Uganda, particularly, in smallholder dairy cattle production. Studies on prioritizing agro-industrial by-products in dairy cattle feeding are central to formulation of sustainable feed management strategies. This study was thus conducted to investigate farmers’ knowledge of agro-industrial by-product management as dairy cattle feed to build logical ideology required in development of appropriate feed management strategies. Focus group discussion and individual interviews were conducted in Lake Victoria Crescent agro-ecological zone to capture information on farmers’ knowledge of the factors enhancing agro-industrial by-products utilization, temporal and spatial variability, and limitations to utilization in smallholder dairy farming system. Chi-square test at p<0.01, df = 3 showed a significant difference among farmers rankings of utilization and factors limiting utilization (p<0.01, df = 4) of agro-industrial by-products across all the study districts. There was no significant difference (p>0.05) in spatial and temporal variability of agro-industrial by-products. The study provided basic information about farmers’ knowledge on importance of agro-industrial by-products that could aid the development of sustainable and socially acceptable dairy cattle feed management strategies.