Browsing by Author "Aharinta, Kenneth Faith"

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    Comparative Phenotypic and Agronomic Assessment of Transgenic Potato with 3R-Gene Stack with Complete Resistance to Late Blight Disease
    (Biology, 2021) Byarugaba, Arinaitwe Abel; Baguma, Gerald; Mutebi Jjemba, Douglas; Aharinta, Kenneth Faith; Wasukira, Arthur; Magembe, Eric; Njoroge, Anne; Barekye, Alex; Ghislain, Marc
    Transgenic potato event Vic.172, expressing three naturally occurring resistance genes (R genes) conferring complete protection against late blight disease, was evaluated for resistance to late blight, phenotypic characterization, and agronomic performance in field conditions at three locations during three seasons in Uganda. These trials were conducted by comparison to the variety Victoria from which Vic.172 derives, using identical fungicide treatment, except when evaluating disease resistance. During all seasons, the transgenic event Vic.172 was confirmed to have complete resistance to late blight disease, whereas Victoria plants were completely dead by 60–80 days after planting. Tubers from Vic.172 were completely resistant to LB after artificial inoculation. The phenotypic characterization included observations of the characteristics and development of the stems, leaves, flowers, and tubers. Differences in phenotypic parameters between Vic.172 and Victoria were not statistically significant across locations and seasons. The agronomic performance observations covered sprouting, emergence, vigor, foliage growth, and yield. Differences in agronomic performance were not statistically significant except for marketable yield in one location under high productivity conditions. However, yield variation across locations and seasons was not statistically significant, but was influenced by the environment. Hence, the results of the comparative assessment of the phenotype and agronomic performance revealed that transgenic event Vic.172 did not present biologically significant differences in comparison to the variety Victoria it derives from.
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    Effectiveness of Chia Seed Plant Powder in Comparison with Malathion in the Control of Bean Weevils
    (East African Nature and Science Organization, 2023-06-22) Kamugisha, Francis; Opio, Fina; Osiru, David; Aharinta, Kenneth Faith
    The primary objective of the research was to contribute to the control of storage pests in beans through the use of chia seed plant powder—an organic control method. The study adopted both descriptive and correlational research designs for data collection and analysis. It comprised: a survey of the chia farmers in Kyabugimbi sub-county, Uganda, for the purpose of assessing their perception concerning control of storage pests in beans through the use of chia seed plant powder; and an experiment for assessing the susceptibility of a variety of beans and pesticide application. The experiment had three bean varieties: NAROBEAN3, NABE16, and KAHURA. The type of pesticide had two treatments and control, that is, chia seed plant powder and malathion, and each had three levels. A control consisted of dishes with bean varieties infested with weevils without any pesticide added. Each set was comprised of control without any treatment. A total of 54 samples were used. These were laid out in a completely randomised design (CRD) and replicated three times. Pesticides were applied at 0%, 50%, and 100% rates of application. Data were collected on the following measurements: Number of infested seeds, number of adult bruchids that emerged, and weight of seeds at the end of the experiment. The ANOVA test results found that the effect of pesticide application on the number of infested seeds, number of weevils that emerged, and weight of bean seeds was significant (P< 0.01), while the variety of beans was not significant. Although malathion had the lowest bean weevil figures and is therefore more effective in controlling bean weevils, it is a synthetic product, and chia seed would be promoted because it is an organic product because of being more friendly to the human body and the environment than malathion, a synthetic pesticide