Browsing by Author "Achaye, Innocent"

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    Phytochemical composition and antioxidant activities of some wild edible plants locally consumed by rural communities in northern Uganda
    (Frontiers in Nutrition, 2023) Nyero, Alfred; Anywar, Godwin Upoki; Achaye, Innocent; Malinga, Geoffrey Maxwell
    Acalypha rhomboidea, Asystacia gangetica, Crassocephalum sacrobasis, Crotalaria ochroleuca, Heterosis rotundifolia, Hibiscus cannabinus, Hibiscus sp., Hibiscus surratensis, Ipomoea eriocarpa, Maerua angolensis, Senna obtusifolia and Vigna membranacea are among the common wild edible plants in the Acholi sub-region, northern Uganda. This study evaluated the phytochemical constituents and antioxidant potential of the plants. Fresh leaves collected from each plant species were air-dried under shade. The phytochemical contents of the ethanol and petroleum ether extracts were determined using standard protocols. The antioxidant content of the methanolic extracts was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Preliminary phytochemical analyses indicated the presence of tannins, reducing compounds, alkaloids, flavonoids, flavons aglycones, flavanosides, anthracenosides, anthocyanosides, volatile oils, coumarins, steroid glycosides, sterols and triterpenes. However, the extracts did not contain any emodols and saponins. The results of the quantitative phytochemical analysis showed that the contents of different phytochemicals detected varied significantly (p < 0.05) among the selected plants. The amount of tannins in mg/g (gallic acid equivalent) of dry weight varied from 3.90 ± 0.16 in C. ochroleuca to 10.41 ± 0.78 in I. eriocarpa, total flavonoids in RE, mg/g dry matter from 4.07 ± 0.11 in I. eriocarpa to 14.94 ± 0.08 in S. obtusifolia. Total alkaloids in mg/100 g ranged from 1.59 ± 0.30 in I. eriocarpa to 6.37 ± 0.24 in Hibiscus sp. Total phenolic content in GAE, mg/g dry matter ranged from 13.39 ± 0.26 in A. rhomboidea to 64.25 ± 0.54 in I. eriocarpa. The in vitro antioxidant assays revealed substantial free radical scavenging activity in all the plants. Antioxidant activity expressed as IC50 (ppm) ranged from 13.39 for A. rhomboidea to 64.84 for I. eriocarpa, compared to 12.82 for ascorbic acid standard. The total phenolic compounds and total tannins had significant and positive correlations with DPPH free radical scavenging activity. The findings of this study provide evidence that the species are good natural sources of phytochemicals and antioxidants, whose regular consumption could provide human health benefits by protecting against oxidative stress related diseases. Further research is needed on the structural characterization of the phytochemicals, profiling the plant extracts with high antioxidant activity and determining the antimicrobial activities.
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    Wild and semi-wild edible plants used by the communities of Acholi sub-region, Northern Uganda
    (Ethnobotany Research and Applications, 2021) Nyero, Alfred; Achaye, Innocent; Odongo, Walter; Anywar, Godwin; Malinga, Geoffrey Maxwell
    In the Acholi sub-region, consumption of wild edible plants is still an integral part of the food culture, particularly during times of food shortage. However, much of indigenous traditional knowledge has not been documented due to the history of prolonged civil war in the area. We conducted an ethnobotanical survey to document the wild plants utilized by the Acholi communities in northern Uganda. Methods: Data was collected using semi-structured questionnaires administered to 1,353 respondents between November 2017 and February 2018 and 65 focus group discussions. Results: Seventy-three edible plants from 39 families were identified to species level while three species were unidentified. Vitex doniana (0.72), Aframomum alboviolaceum. (0.70), Saba comorensis (0.45), Hibiscus surattensis (0.42), and Borassus aethiopum (0.39) had the highest relative frequencies of citation. Fabaceae (7) and Euphorbiaceae (5) had the highest number of plant species. Most of the edible plants were herbs (34%) and trees (31%). Fruits were the major parts consumed (57%), followed by leaves (37%). Only 34% of species were cooked before eating, while 60% were eaten raw. Sixty-seven percent of the species were harvested during the rainy season and 22% in the dry season. Conclusion: There is a rich diversity of wild edible plants in the Acholi sub-region. Conservation and domestication of these plants will provide a buffer crop and enhance the food security of the locals in the face of recurrent droughts and climate change.