Browsing by Author "Abubakar, Abdulhakim"

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    Antinociceptive activity of methanol extract of Chlorophytum alismifolium tubers in murine model of pain: Possible involvement of a2-adrenergic receptor and KATP channels
    (Journal of Traditional and Complementary Medicine, 2020) Abubakar, Abdulhakim; Balarabe Nazifi, Abdullahi; Odoma, Saidi; Shehu, Salisu; Danjuma, Nuhu Mohammed
    The tubers of Chlorophytum alismifolium are used in Nigerian Herbal Medicine for the management of diabetes mellitus, painful and inflammatory conditions. The antinociceptive activity has been validated but the mechanism of this activity is yet to be explored. This study therefore, aimed to investigate the probable mechanism(s) of the antinociceptive activity of C. alismifolium tubers using experimental animal model of pain. HPLC and GC-MS analyses were carried out on the extract. Antinociceptive activity was investigated using acetic acid-induced writhing response test in mice. Three groups of mice were orally administered distilled water (10ml/kg), C. alismifolium (400 mg/kg) and morphine (10 mg/kg) 60 min before administration of acetic acid and the resulting writhing were counted for 10 min. To establish the probable mechanism(s) of action of C. alismifolium, separate groups of animals were pretreated intraperitoneally with naloxone (2 mg/kg), prazosin (1 mg/kg), yohimbine (1 mg/kg), propranolol (20 mg/kg) and glibenclamide (5 mg/kg) 15 min before C. alismifolium administration. HPLC chromatogram of the extract revealed seventeen characteristic peaks with retention times ranging between 2.1 and 7.4min. Administration of C. alismifolium significantly (p < 0.01) reduced the mean number of writhes compared to control group. Pretreatment with yohimbine and glibenclamide significantly (p < 0.05 and p < 0.01 respectively) reduced the antinociceptive activity of extract-alone treated group. However, pretreatment with prazosin, naloxone and propranolol showed no effect on its analgesic activity. The findings from this research revealed the possible involvement of a2-adrenergic receptor and KATP channels in the antinociceptive activity of Chlorophytum alismifolium tuber extract.
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    Antinociceptive and anti-inflammatory activities of the methanol extract of Chlorophytum alismifolium tubers
    (Journal of Traditional and Complementary Medicine, 2021) Abubakar, Abdulhakim; Danjuma, Nuhu M.; Odoma, Saidi; Nazifi, Abdullahi B.
    The tubers of Chlorophytum alismifolium Baker (Family: Liliaceae) are used in Herbal Medicine for the management of various ailments including diabetes mellitus, erectile dysfunction, abdominal pains and inflammatory conditions. Despite its wide usage for management of pain and inflammation, there is no scientific justification to validate this claim. This study was aimed at screening the methanol tuber extract of Chlorophytum alismifolium for antinociceptive and anti-inflammatory activities using experimental animal models. The antinociceptive activity was tested using acetic acid-induced writhing response in Swiss albino mice and formalininduced pain in Wistar rats, while the anti-inflammatory activity was tested using carrageenan-induced paw edema in rats at doses of 200, 400 and 800 mg/kg. The extract significantly (p< 0.001) reduced the number of writhes at all tested doses. At 800 mg/kg, it significantly (p< 0.01) inhibited the first phase of formalin-induced pain while the second phase was inhibited significantly (p< 0.05 and p< 0.01) at doses of 400 and 800 mg/kg respectively. The extract (400 and 800 mg/kg) significantly (p< 0.05) inhibited the carrageenan-induced inflammation at the third hour. A similar activity was also observed at the fourth hour with 61.61% inhibition of paw oedema at 400 mg/kg. These findings suggest that Chlorophytum alismifolium tuber extract possesses antinociceptive and antiinflammatory activities, thus support the claim for the ethnomedical use of the plant in the management of pain and inflammatory diseases.