Agnihotri, NaveenKisaalita, William S.Keith, Charles H.2025-04-162025-04-161999Agnihotri, N., Kisaalita, W. S., & Keith, C. H. (1999). Micro-perfusion flow cell for imaging cultured cells. Biotechniques, 27(4), 722-728.https://doi.org/10.2144/99274st01https://doi.org/10.2144/99274st01https://nru.uncst.go.ug/handle/123456789/10808We present a unique design for a flow cell with a small working volume that allows rapid displacement of media viewed under high power and short working distance objectives. The flow cell has a small internal depth (ca. 0.033 cm) and volume (ca. 0.05 mL) and is easy to handle. Made of Delrin®, the flow cell is biologically inert. We have used the flow cell for fluorescence imaging of PC12 cells loaded with tetramethylrhodamine dextran (TMRD) and other dyes.enArticle