Papastergiadis, AntoniosMubiru, EdwardVan Langenhove, HermanDe Meulenaer, Bruno2023-06-142023-06-142014Papastergiadis, A., Mubiru, E., Van Langenhove, H., & De Meulenaer, B. (2014). Development of a sensitive and accurate stable isotope dilution assay for the simultaneous determination of free 4-hydroxy-2-(E)-nonenal and 4-hydroxy-2-(E)-hexenal in various food matrices by gas chromatography–mass spectrometry. Food analytical methods, 7, 836-843. DOI 10.1007/s12161-013-9689-210.1007/s12161-013-9689-2https://nru.uncst.go.ug/handle/123456789/8926An analytical method suitable for the determination of 4-hydroxy-2-(E)-nonenal (HNE) and 4-hydroxy-2-(E)- hexenal (HHE) in various food matrices was developed and validated. The method involves the use of deuterated HNE and HHE as internal standards, extraction of the analytes from the matrices followed by derivatization and detection with gas chromatography–mass spectrometry. Four different food ma- trices were chosen as model systems including vegetable oils, unprocessed meat, fried potato crisps, and infant formula and three different extraction techniques suitable for the different matrices were applied including the Quick Easy Cheap Effec- tive Rugged Safe method. The simplicity of the extraction techniques allows the method to be applied for routine anal- ysis of a large amount of samples. The results verify the accuracy and reproducibility of the analytical technique and its ability to provide reliable quantification of both analytes at concentrations as low as 12.8 ng g−1 in meat samples. Fur- thermore, a short overview of the levels of HNE and HHE in several products available in the Belgian market is presented.enLipid oxidation4-Hydroxy-2-(E)-nonenalHydroxy-2-(E)-hexenalQuEchERSArticle