Galiwango, Ronald M.Musoke, RichardLubyayi, LawrenceSsekubugu, RobertKalibbala, SarahSsekweyama, ViolaMirembe, ViolaNakigozi, GertrudeReynolds, Steven J.Serwadda, DavidGray, Ronald H.Kigozi, Godfrey2022-01-202022-01-202013Galiwango, R. M., Musoke, R., Lubyayi, L., Ssekubugu, R., Kalibbala, S., Ssekweyama, V., ... & Kigozi, G. (2013). Evaluation of current rapid HIV test algorithms in Rakai, Uganda. Journal of virological methods, 192(1-2), 25-27. doi:10.1016/j.jviromet.2013.04.003.10.1016/j.jviromet.2013.04.003.https://nru.uncst.go.ug/xmlui/handle/123456789/1363Rapid HIV tests are a crucial component of HIV diagnosis in resource limited settings. In Uganda, the Ministry of Health allows for both serial and parallel HIV rapid testing using Determine, Stat- Pak and Uni-Gold. In serial testing, a non-reactive result on Determine ends testing. The performance of serial and parallel algorithms with Determine and Stat-Pak test kits was assessed. A cross-sectional diagnostic test accuracy evaluation using three rapid HIV test kits as per the recommended parallel test algorithm was followed by EIA-WB testing with estimates of the performance of serial testing algorithm. In 2520 participants tested by parallel rapid algorithms, 0.6% had weakly reactive result. Parallel testing had 99.7% sensitivity and 99.8% specificity. If Stat-Pak was used as the first screening test for a serial algorithm, the sensitivity was 99.6% and specificity 99.7%. However, if Determine was used as the screening test, sensitivity was 97.3% and specificity 99.9%. Serial testing with Stat-Pak as the initial screening test performed as well as parallel testing, but Determine was a less sensitive screen. Serial testing could be cost saving.enHIV test algorithmsRakaiUgandaArticle