Chepkirui, ClaraMatasyoh, Josphat C.Wagara, Isabel N.Nakavuma, Jesca2022-12-132022-12-132014Chepkirui Clara, Josphat C. Matasyoh, Isabel N. Wagara, Jesca Nakavuma. Antifungal Activity of Flavonoids Isolated from Monanthotaxis littoralis against Mycotoxigenic Fungi from Maize. American Journal of Chemistry and Application. Vol. 1, No. 4, 2014, pp. 54-60.https://www.academia.edu/download/35028380/new_paper.pdfhttps://nru.uncst.go.ug/handle/123456789/6227This study evaluated the antifungal activity of a mixture of two closely related flavonoids namely 3, 7, 5’- trihydroxy anthocynidines and 3, 5-dihydroxy-7-methoxy anthocynidines isolated from Monanthotaxis littoralis (Annonaceae) against mycotoxigenic fungi from three genera (Aspergillus, Fusarium and Penicillium) isolated from maize samples. Dry leaves of Monanthotaxis littoralis was extracted with methanol, suspended in water and extracted with ethyl acetate. Column chromatography and Preparative Thin Layer Chromatography (PTLC) was used to purify the compounds. 1D and 2D NMR and Mass Spectroscopy were used to elucidate the structures of the compounds isolated. The extent of inhibition of fungal growth was dependent on the concentration of the flavonoids. The highest activity of the flavonoids was that against Aspergillus ochraceus with inhibition zone of 20.17 mm and MIC of 1 mg/ml. The activity of the flavonoids against the mycotoxigenic fungi had Minimum Inhibitory Concentration (MIC) values ranging from 1 mg/ml to 4 mg/ml. These results show that the two flavonoids from M. littoralis have antifungal activities against fungi that are the producers of poisonous mycotoxins found in foods. These compounds are potential antimicrobials that can be used in food preservation systems to inhibit the growth of moulds and retard subsequent mycotoxin productionenAntifungalflavonoidsArticle