SSENYONGA, G. S. Z.KAKOMA, I.MONTENEGRO-JAMES, S.NYEKO, P. J.NANTEZA, A.BUGA, R.2023-03-072023-03-071992Ssenyonga, G. S. Z., Kakoma, I., MONTENEGRO‐JAMES, S., Nyeko, P. J., Nanteza, A., & Buga, R. (1992). Anaplasmosis in Uganda. II. Prevalence of bovine anaplasmosis. Scandinavian Journal of Immunology, 36, 107-109.https://doi.org/10.1111/j.1365-3083.1992.tb01631.xhttps://nru.uncst.go.ug/handle/123456789/8124The prevalence of bovine anaplasmosis was studied in 320 Zebu cattle randomly selected from three regions of Uganda (central, south-western and north-western) using DOT-ELISA, Western immunoblotting. Rapid Card Agglutination Test (RCAT), Capillary Tube Agglutination Test (CAT), Complement Fixation Test (CFT), and parasitological techniques. Dried blood on Whatman filter paper no. I was eluated in PBS 0.05% Tween 20 prior to testing at an initial dilution of 1:25. The incidence of parasitaemia ranged from 25% in the central region to 35% in the northwestern region and the serological prevalence was lower in the central region and highest in the north-west. Prevalence rates assayed by DOT-ELISA and Western immunoblotting were 1.5-fold greater than those tested with RCAT and 3-fold greater than in CAT. The overall prevalence rates by DOT-ELISA and Western immunoblotting compared favourably with CFT data. The present data utilizing dried blood on filter papers indicate that there is a high prevalence of anaplasmosis in those regions of Uganda surveyed and it confirms our observations and those of others that collecting blood on filter papers is a suitable technique for large-scale screening and for seroepidemiological studies.enAnaplasmosisBovine AnaplasmosisArticle