Trinha, Dai QuangOgawa, HarukoNghia Bui, VuongThi Hong Nguyen, ThamGronsang, DulyatadBaatartsogt, TugsbaatarKahoza Kizito, MugimbaAbo Elkhair, MohammedYamaguchi, ShigeoNguyen, Viet KhongImai, Kunitoshi2023-01-242023-01-242015Ogawa, H., Bui, V. N., Nguyen, T. T. H., Gronsang, D., Baatartsogt, T., Kizito, M. K., ... & Imai, K. (2015). Development of a blocking latex agglutination test for the detection of antibodies to chicken anemia virus. Journal of virological methods, 221, 74-80. http://dx.doi.org/10.1016/j.jviromet.2015.04.027http://dx.doi.org/10.1016/j.jviromet.2015.04.027https://nru.uncst.go.ug/handle/123456789/7185tA blocking latex agglutination test (b-LAT) developed in this study was evaluated for the detection ofantibodies against chicken anemia virus (CAV) in chickens. Polystyrene latex beads were coupled witha neutralizing monoclonal antibody (mAb) to CAV (mAb-beads). When mAb-beads were mixed withantigens prepared from the lysate of MDCC-MSB1 cells infected with CAV, agglutination occurred. A shortpre-incubation of CAV antigens with CAV-specific antiserum inhibited the agglutination of mAb-beads.The test results were obtained within 5 min. The specificity of b-LAT was evaluated using sera from specificpathogen-free chickens and sera containing antibodies to avian influenza virus, Newcastle disease virus,infectious bursal disease virus, and Marek’s disease virus; nonspecific agglutination and cross-reactivitywith antibodies to unrelated viruses were not observed. The examination of 94 serum samples collectedfrom commercial breeder chickens of various ages (17–63 weeks) revealed good agreement (93.6%, Kappavalue = 0.82) between b-LAT and a virus neutralization test, known to be most sensitive and specific inthe detection of antibodies to CAV. These results indicate that b-LAT, a simple and rapid test, is a usefuland reliable tool in CAV serology.enChicken anemia virusSerum antibodyBlocking latex agglutination testMonoclonal antibodyArticle