Wayengera, MisakiKajumbula, HenryByarugaba, Wilson2021-12-122021-12-122008Wayengera, M., Kajumbula, H., & Byarugaba, W. (2008). Identification of restriction endonuclease with potential ability to cleave the HSV-2 genome: inherent potential for biosynthetic versus live recombinant microbicides. Theoretical Biology and Medical Modeling , 5 (1), 1-12. doi:10.1186/1742-4682-5-1810.1186/1742-4682-5-18https://nru.uncst.go.ug/xmlui/handle/123456789/378Herpes Simplex virus types 1 and 2 are enveloped viruses with a linear dsDNA genome of ~120–200 kb. Genital infection with HSV-2 has been denoted as a major risk factor for acquisition and transmission of HIV-1. Developing biomedical strategies for HSV-2 prevention is thus a central strategy in reducing global HIV-1 prevalence. This paper details the protocol for the isolation of restriction endonucleases (REases) with potent activity against the HSV-2 genome and models two biomedical interventions for preventing HSV-2.enEndonucleaseHSV-2 genomeBiosyntheticMicrobicidesArticle