Byarugaba, Wilson2022-06-062022-06-061978Byarugaba, W. (1978). A pepsin-revealed material possibly related to chromosomal banding. Chromosoma, 67(3), 275-283.https://link.springer.com/article/10.1007/BF02569040https://nru.uncst.go.ug/handle/123456789/3761The enzymes pepsin, c~-chymotrypsin, trypsin, RNase and DNase were applied to preparations of human metaphase chromosomes before staining to study whether dissociable materials related to the formation of G-, Q- and C-bands would be seen. Treatment with active pepsin but not the other enzymes revealed material with ribonucleo-protein properties which dissociated from the chromosomes and formed a halo. - Lateral extensions from the chromatids stretched to the rim of the halo and appeared at positions corresponding to G-bands. A G-band may be defined as a ring of stable chromatid-matrix binding at positions where the chromatids coil to form lateral extensions.enPepsin-Revealed MaterialChromosomal BandingArticle