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    Expression of a rice chitinase gene in transgenic banana (‘Gros Michel’, AAA genome group) confers resistance to black leaf streak disease

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    Expression of a rice chitinase gene in transgenic banana (‘Gros Michel’, AAA genome group) confers resistance to black leaf streak disease (504.4Kb)
    Date
    2013
    Author
    Kova´cs, Gabriella
    Sa´gi, La´szlo´
    Jacon, Ge´raldine
    Arinaitwe, Geofrey
    Busogoro, Jean-Pierre
    Thiry, Els
    Strosse, Hannelore
    Swennen, Rony
    Remy, Serge
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    Abstract
    Transgenic banana (Musa acuminata ‘Gros Michel’) integrating either of two rice chitinase genes was generated and its resistance to Black Leaf Streak disease caused by the fungus Mycosphaerella fijiensis was tested using a leaf disk bioassay. PCR screening indicated the presence of the hpt selectable marker gene in more than 90 % of the lines tested, whereas more than three quarters of the lines contained the linked rice chitinase gene resulting in a co-transformation frequency of at least 71.4 %. Further, a unique stable integration of the transgenes in each line revealed some false negative PCR results and the expected co-transformation frequency of 100 %. Gabriella Kova´cs, La´szlo´ Sa´gi contributed equally to this work.
    URI
    https://nru.uncst.go.ug/xmlui/handle/123456789/750
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    • Agricultural and Veterinary Sciences [1451]

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