In vitro somatic embryogenesis and regeneration potential of two potato varieties in Uganda
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Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
African Journal of Biotechnology
Abstract
In vitro somatic embryogenesis and regeneration are important techniques for crop improvement and
mass propagation. This study was conducted to establish and optimize a regeneration system for
agronomically important potato cultivars (‘Victoria’ and ‘Rutuku’) of Solanum tuberosum L. in Uganda.
Completely randomized design experiments were set up at Bioscience East and Central Africa (BecA)
Tissue Culture Laboratory for this purpose. Callus induction and plant regeneration were initiated on
internodes and leaf segments of the two potato cultivars in vitro on Murashige and Skoog (MS) medium
supplemented with different phytohormones that included auxins such as α naphthalene acetic acid
(NAA), cytokinins like benzyl amino purine (BAP), gibberellic acid (GA3), 2,4-dichlorophenoxyacetic acid
(2,4-D) and Zeatin at varying concentrations. Callus response depended on the genotype, the
concentrations and composition of growth substances. Hormone combination MS + Sucrose 30% +
NAA (2 mg/l) + 2,4-D (2 mg/l) + KN (2 mg/l) was found to have the highest callusing rate, that is, 95 and
53%, respectively for internodes and leaf explants. The calli formed from internodes and leaves were
friable and soft. The callus colour in all cases ranged from light brown to light cream and in some cases
to light green. Shoot bud initiation was observed in all regeneration culture media, with media
combination MS + KN (2 mg/l) + Zeatin (2 mg/l) giving the highest shooting rate. The intervening callus
phase led to less number of shoot buds for each callus leading to long incubation period. The study
showed that it is possible to regenerate potato cultivars (‘Victoria’ and ‘Rutuku’) from cell suspension
culture using Murashige and Skoog (MS) medium supplemented with different phytohormones.
Combination of MS with NAA + 2,4-D + Kinetin is suitable for callus induction while MS + kinetin and
zeatin was better for shoot induction on calli of potato cultivars ‘Victoria’ and ‘Rutuku’.
Description
Keywords
Regeneration, Somatic embryogenesis, Solanum tuberosum.
Citation
Byarugaba, A. A., Eric, M., Alex, B., & Skilton, R. (2018). In vitro somatic embryogenesis and regeneration potential of two potato varieties in Uganda. African Journal of Biotechnology, 17(15), 515-520. DOI: 10.5897/AJB2018.16431