Browsing by Author "Gumbi, Bhekumuzi P."

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    Assessment of nonsteroidal anti-inflammatory drugs by ultrasonic-assisted extraction and GC-MS in Mgeni and Msunduzi river sediments, KwaZulu-Natal, South Africa
    (Environmental Science and Pollution Research, 2017) Gumbi, Bhekumuzi P.; Moodley, Brenda; Birungi, Grace; Ndungu, Patrick G.
    The occurrence of eight pharmaceuticals was monitored during four seasons (spring, summer, autumn, and winter) along a 250-km stretch of the Msunduzi and Mgeni rivers in KwaZulu-Natal, South Africa. This paper describes an optimized method for the determination of nonsteroidal antiinflammatory drugs (NSAIDs) in sediments. The method combines ultrasonic, centrifuge, and gas chromatographymass spectrometry for the detection of these drugs in solid samples. Most of the parameters that affect the extraction step were optimized. Solid samples were placed in a centrifuge tube and extracted with ethyl acetate:acetone (1:1, two cycles), followed by clean-up with Oasis HLB cartridge and derivatization with N, O-bis(trimethylsilyl) trifluoroacetamide (BSTFA). Satisfactory recoveries were obtained ranging from 66 to 130%, depending on the analyte. Precision expressed as RSD (%) (n = 3) was less than 20%for all analytes. The LODs and LOQs were in the range of 0.024 to 1.90 ng g−1 which allowed to be applied in the analysis solid samples in Msunduzi and Mgeni rivers. In the solid samples analyzed, NSAID concentration ranged from not detected to 221 ng g−1.
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    Detection and quantification of acidic drug residues in South African surface water using gas chromatography-mass spectrometry
    (Chemosphere, 2017) Gumbi, Bhekumuzi P.; Moodley, Brenda; Birungi, Grace; Ndungu, Patrick G.
    A method was optimized for derivatization, separation, detection and quantification of salicylic acid, acetylsalicylic acid, nalidixic acid, ibuprofen, phenacetin, naproxen, ketoprofen, meclofenamic acid and diclofenac in surface water using gas chromatography-mass spectrometry. For most of the acidic drugs, recovery was in the range 60e110% and the percent standard deviation was below 15% for the entire method, with limits of detection ranging from 0.041 to 1.614 mg L-1. The developed method was applied in the analysis of acidic drugs in Umgeni River system, KwaZulu-Natal South Africa. All of the selected acidic drugs were detected and quantified, their concentration in Umgeni River system ranged from 0.0200 to 68.14 mg L-1.